Human Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) DIY ELISA Kit | RANkL diy elisa kit

Human Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) ELISA Kit DIY Materials

Cat. Num. AAA2088877

• Gene Names: TNFSF11; ODF; OPGL; sOdf; CD254; OPTB2; RANKL; TNLG6B; TRANCE; hRANKL2
• Reactivity: Human
• Synonyms: Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL); Human Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) ELISA Kit DIY Materials; CD254; TNFSF11; ODF; OPGL; TRANCE; HRANKL2; SOdf; Tumor Necrosis Factor(ligand)superfamily Member 11; TNF-related activation-induced cytokine; RANkL diy elisa kit

• Reactivity: Human
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL). No significant cross-reactivity or interference between Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) and analogues was observed.
• Sequence Length: 317

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Double-antibody Sandwich ELISA for Antigen Detection
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.055ng/mL
• Reagent Contents: Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for RANkL diy elisa kit

Signal transduction; Metabolic pathway; Apoptosis; Tumor immunity; Bone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 18143619
• NCBI GeneID: 8600
• Molecular Weight: 30,523 Da
• NCBI Official Full Name: receptor activator of nuclear factor kappa B ligand 1
• NCBI Official Synonym Full Names: tumor necrosis factor superfamily member 11
• NCBI Official Symbol: TNFSF11
• NCBI Official Synonym Symbols: ODF; OPGL; sOdf; CD254; OPTB2; RANKL; TNLG6B; TRANCE; hRANKL2
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 11
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 11
• UniProt Gene Name: TNFSF11
• UniProt Synonym Gene Names: OPGL; RANKL; TRANCE; ODF; OPGL; RANKL; TRANCE

NCBI Description

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found. [provided by RefSeq, Jul 2008]

Uniprot Description

TNFSF11: Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Homotrimer. Up-regulated by T-cell receptor stimulation. Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid. Belongs to the tumor necrosis factor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 13q14.11

Cellular Component: extracellular region; plasma membrane

Molecular Function: cytokine activity; tumor necrosis factor receptor superfamily binding

Biological Process: activation of NF-kappaB transcription factor; calcium-mediated signaling; cytokine and chemokine mediated signaling pathway; monocyte chemotaxis; osteoclast differentiation; positive regulation of bone resorption; positive regulation of corticotropin-releasing hormone secretion; positive regulation of homotypic cell-cell adhesion; positive regulation of MAP kinase activity; positive regulation of osteoclast differentiation; positive regulation of T cell activation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; tumor necrosis factor-mediated signaling pathway

Disease: Osteopetrosis, Autosomal Recessive 2