Western Blot (WB)
(Western blot analysis in CEM cell line lysates (35ug/lane) using MBS6008017. PIK3R1(arrow) was detected using the purified Pab.)
Rabbit anti-Human PI3KR1 Polyclonal Antibody | anti-SRC antibody
PI3KR1, NT (L11) (PIK3R1, GRB1, Phosphatidylinositol 3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85kD regulatory subunit alpha)
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-100
Immunohistochemistry: 1:10-50
Immunoprecipitation Not tested
Flow cytometry Not tested
Western Blot (WB)
(Western blot analysis in CEM cell line lysates (35ug/lane) using MBS6008017. PIK3R1(arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis in CEM cell line lysates (35ug/lane) using MBS6008017. PIK3R1(arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis in Hela cell line lysates (35ug/lane) using MBS6008017. PIK3R1(arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis in Hela cell line lysates (35ug/lane) using MBS6008017. PIK3R1(arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis in 293 cell line lysates transiently transfected with the PIK3R1 gene (3ug/lane) using MBS6008017. PI3KR1(arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis in 293 cell line lysates transiently transfected with the PIK3R1 gene (3ug/lane) using MBS6008017. PI3KR1(arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with MBS6008017, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with MBS6008017, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
NCBI and Uniprot Product Information
NCBI Description
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Src: proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme. Two alternatively spliced isoforms have been described.
Protein type: Oncoprotein; Kinase, protein; Protein kinase, TK; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); TK group; Src family
Chromosomal Location of Human Ortholog: 20q12-q13
Cellular Component: neuron projection; mitochondrion; lysosome; postsynaptic density; actin filament; caveola; cytosol; extrinsic to internal side of plasma membrane; perinuclear region of cytoplasm; mitochondrial inner membrane; cytoplasm; late endosome; plasma membrane; nucleus
Molecular Function: protein C-terminus binding; ephrin receptor binding; non-membrane spanning protein tyrosine kinase activity; phosphoprotein binding; insulin receptor binding; protein kinase activity; integrin binding; protein binding; enzyme binding; SH3/SH2 adaptor activity; protein kinase C binding; protein-tyrosine kinase activity; heme binding; estrogen receptor binding; kinase activity; SH2 domain binding; ATP binding; receptor binding; hormone receptor binding
Biological Process: oogenesis; regulation of estrogen receptor signaling pathway; positive regulation of cyclin-dependent protein kinase activity; central nervous system development; estrogen receptor signaling pathway; progesterone receptor signaling pathway; nerve growth factor receptor signaling pathway; viral reproduction; positive regulation of apoptosis; regulation of cell cycle; positive regulation of transcription, DNA-dependent; uterus development; negative regulation of mitochondrial depolarization; negative regulation of protein homooligomerization; positive regulation of MAP kinase activity; cell-cell adhesion; transforming growth factor beta receptor signaling pathway; cell adhesion; response to electrical stimulus; bone resorption; response to drug; platelet activation; fibroblast growth factor receptor signaling pathway; activation of protein kinase B; response to virus; transcytosis; positive regulation of protein amino acid autophosphorylation; positive regulation of integrin activation; cellular response to insulin stimulus; response to mechanical stimulus; T cell costimulation; regulation of vascular permeability; negative regulation of transcription, DNA-dependent; leukocyte migration; negative regulation of apoptosis; axon guidance; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; platelet-derived growth factor receptor signaling pathway; negative regulation of caspase activity; positive regulation of smooth muscle cell migration; signal transduction; regulation of cell-cell adhesion; forebrain development; ephrin receptor signaling pathway; response to nutrient levels; integrin-mediated signaling pathway; epidermal growth factor receptor signaling pathway; regulation of bone resorption; negative regulation of focal adhesion formation; positive regulation of phosphoinositide 3-kinase activity; signal complex assembly; response to mineralocorticoid stimulus; cell cycle; regulation of cell proliferation; positive regulation of protein kinase B signaling cascade; cell proliferation; peptidyl-serine phosphorylation; response to hydrogen peroxide; regulation of protein binding; Ras protein signal transduction; stress fiber formation; innate immune response; positive regulation of insulin receptor signaling pathway; response to acidity; blood coagulation; vascular endothelial growth factor receptor signaling pathway; positive regulation of cytokine secretion
Research Articles on SRC
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Product Notes
The SRC src (Catalog #AAA6008017) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PI3KR1, NT (L11) (PIK3R1, GRB1, Phosphatidylinositol 3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85kD regulatory subunit alpha) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PI3KR1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot, Immunohistochemistry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:50-100 Immunohistochemistry: 1:10-50 Immunoprecipitation Not tested Flow cytometry Not tested. Researchers should empirically determine the suitability of the SRC src for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PI3KR1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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