Human Juxtaposed With Another Zinc Finger Protein 1 (JAZF1) ELISA Kit | JAZF1 elisa kit

Human Juxtaposed With Another Zinc Finger Protein 1 (JAZF1) ELISA Kit

Cat. Num. AAA2088204

• Gene Names: JAZF1; TIP27; ZNF802
• Reactivity: Human
• Synonyms: Juxtaposed With Another Zinc Finger Protein 1 (JAZF1); Human Juxtaposed With Another Zinc Finger Protein 1 (JAZF1) ELISA Kit; TIP27; ZNF802; TAK1-interacting protein 27; Zinc finger protein 802; JAZF1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Juxtaposed With Another Zinc Finger Protein 1 (JAZF1).; No significant cross-reactivity or interference between Juxtaposed With Another Zinc Finger Protein 1 (JAZF1) and analogues was observed.
• Sequence Length: 243

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.058ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Juxtaposed With Another Zinc Finger Protein 1 (JAZF1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Juxtaposed With Another Zinc Finger Protein 1 (JAZF1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for JAZF1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Juxtaposed With Another Zinc Finger Protein 1 (JAZF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Juxtaposed With Another Zinc Finger Protein 1 (JAZF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Juxtaposed With Another Zinc Finger Protein 1 (JAZF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Juxtaposed With Another Zinc Finger Protein 1 (JAZF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for JAZF1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 91822911
• NCBI GeneID: 221895
• NCBI Accession #: NP_778231.2
• NCBI GenBank Nucleotide #: NM_175061.3
• UniProt Accession #: Q86VZ6; Q8N3L7; A4D195
• Molecular Weight: 25,739 Da
• NCBI Official Full Name: juxtaposed with another zinc finger protein 1
• NCBI Official Synonym Full Names: JAZF zinc finger 1
• NCBI Official Symbol: JAZF1
• NCBI Official Synonym Symbols: TIP27; ZNF802
• NCBI Protein Information: juxtaposed with another zinc finger protein 1
• UniProt Protein Name: Juxtaposed with another zinc finger protein 1
• Protein Family: Juxtaposed with another zinc finger protein
• UniProt Gene Name: JAZF1
• UniProt Synonym Gene Names: TIP27; ZNF802

NCBI Description

This gene encodes a nuclear protein with three C2H2-type zinc fingers, and functions as a transcriptional repressor. Chromosomal aberrations involving this gene are associated with endometrial stromal tumors. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized [provided by RefSeq, Jul 2008]

Uniprot Description

JAZF1: Potential transcription factor. A chromosomal aberration involving JAZF1 may be a cause of endometrial stromal tumors. Translocation t(7;17)(p15;q21) with SUZ12. The translocation generates the JAZF1-SUZ12 oncogene consisting of the N-terminus part of JAZF1 and the C-terminus part of SUZ12. It is frequently found in all cases of endometrial stromal tumors, except in endometrial stromal sarcomas, where it is rarer. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; Nuclear receptor co-regulator; Oncoprotein; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 7p15.2-p15.1

Cellular Component: cytoplasm; nucleolus; nucleus; transcriptional repressor complex

Molecular Function: transcription corepressor activity

Biological Process: negative regulation of transcription from RNA polymerase II promoter