Mouse anti-Human KCTD15 Monoclonal Antibody | anti-KCTD15 antibody

KCTD15 antibody

Cat. Num. AAA206581

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry
• Purity: By protein-G affinity chromatography
• Synonyms: KCTD15; Monoclonal Antibody; KCTD15 antibody; BTB/POZ domain-containing protein KCTD15; MGC25497; MGC2628; anti-KCTD15 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b, k
• Clone Number: AT2B11
• Purity/Purification: By protein-G affinity chromatography
• Form/Format: Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 234

• Applicable Applications for anti-KCTD15 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC)
• Immunogen: Recombinant human KCTD15 (1-159aa) purified from E. coli.
• Usage: The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
• Preparation and Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Western Blot (WB)

(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KCTD15 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1 : HeLa cell lysateLane 2 : NIH/3T3 cell lysateLane 3 : K562 cell lysate)

Immunofluorescence (IF)

(ICC/IF analysis of KCTD15 in HeLa cells. The cell was stained with MBS206581 (1:100). The secondary antibody (green) was used Alexa Fluor 488. )

Flow Cytometry (FC)

(Flow cytometry analysis of KCTD15 in HeLa cells. The cell was stained with ATGA0121 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).)

Related Product Information for anti-KCTD15 antibody

KCTD15 (Potassium channel tetramerisation domain containing 15, also known as BTB/POZ domain-containing protein KCTD15) is protein that in humans is encoded by the KCTD15 gene. KCTD15 is expressed at high level in brain and hypothalamus. The potassium channel KCTD15 was identified as a genetic loci associated with higher than normal body mass index (BMI) in humans along with genes such as GNPDA2, MTCH2, FTO, and TMEM18. Single nucleotide polymorphisms (SNPs) in non-diabetic and diabetic patients showed that FTO was most strongly associated with obesity while MTCH2 and GNPDA2 were still significantly associated with higher than normal BMI levels.

Product Categories/Family for anti-KCTD15 antibody

Neuroscience

References

Elks CE, et al. (2010) PLoS Med. 7 (5): e1000284.; Strausberg RL, et al. (2002) Proc. Natl. Acad. Sci. U.S.A, 99 (26): 16899-903.; Willer CJ, et al. (2010) Nat. Genet, 41 (1): 24-34.

NCBI and Uniprot Product Information

• NCBI GI #: 194018439
• NCBI GeneID: 79047
• NCBI Accession #: NP_076981
• NCBI GenBank Nucleotide #: NM_024076.2
• UniProt Accession #: Q96SI1; Q9BVI6; A8K600
• NCBI Official Full Name: BTB/POZ domain-containing protein KCTD15 isoform 1
• NCBI Official Synonym Full Names: potassium channel tetramerization domain containing 15
• NCBI Official Symbol: KCTD15
• NCBI Protein Information: BTB/POZ domain-containing protein KCTD15
• UniProt Protein Name: BTB/POZ domain-containing protein KCTD15
• Protein Family: BTB/POZ domain-containing protein
• UniProt Gene Name: KCTD15

Uniprot Description

During embryonic development, interferes with neural crest formation (). Inhibits AP2 transcriptional activity by interaction with its activation domain.