LIF active protein

LIF, 23-202aa, Human, His-tag, Baculovirus (Bioactivity Validated)

Cat. Num. AAA206539

• Gene Names: LIF; CDF; DIA; HILDA; MLPLI
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: LIF; 23-202aa; Human; His-tag; Baculovirus (Bioactivity Validated); Leukemia inhibitory factor isoform1; CDF; DIA; HILDA; MLPLI; LIF active protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: ADPSPLPITP VNATCAIRHP CHNNLMNQIR SQLAQLNGSA NALFILYYTA QGEPFPNNLD KLCGPNVTDF PPFHANGTEK AKLVELYRIV VYLGTSLGNI TRDQKILNPS ALSLHSKLNA TADILRGLLS NVLCRLCSKY HVGHVDVTYG PDTSGKDVFQ KKKLGCQLLG KYKQIIAVLA QAFHHHHHH
• Sequence Length: 202

• Applicable Applications for LIF active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Biological Activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 for this effects is less or equal to 0.5 ng/ml.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for LIF active protein

LIF, as known as leukemia inhibitory factor, is a pleiotropic glycoprotein belonging to the LI6 family of cytokines. This protein is involved in growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, embryogenesis and inflammation. It is produced by the adrenal cortex and likely enhances its production of cortisol and aldosterone. Also, it can function as an autocrine growth factor in some pancreatic cancers, but induced differentiation in the leukemic cell line M1. Recombinant human LIF, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for LIF active protein

Immunology; Hormones & Cytokines

References

Kuphal S., et al, (2013) Exp. Mol. Pathol. 95:156-165.; Liu SC., et al, (2013) J. Clin. Invest. 123:5269-5283.

NCBI and Uniprot Product Information

• NCBI GI #: 4504991
• NCBI GeneID: 3976
• NCBI Accession #: NP_002300
• NCBI GenBank Nucleotide #: NM_002309.4
• UniProt Accession #: P15018; Q52LZ2; B2RCW7; B5MC23
• Molecular Weight: 20.8kDa (189aa) 18-40kDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: leukemia inhibitory factor isoform 1
• NCBI Official Synonym Full Names: LIF, interleukin 6 family cytokine
• NCBI Official Symbol: LIF
• NCBI Official Synonym Symbols: CDF; DIA; HILDA; MLPLI
• NCBI Protein Information: leukemia inhibitory factor
• UniProt Protein Name: Leukemia inhibitory factor
• Protein Family: Leukemia inhibitory factor
• UniProt Gene Name: LIF
• UniProt Synonym Gene Names: HILDA; LIF; D factor; MLPLI

NCBI Description

The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]

Uniprot Description

LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.