PGC recombinant protein

PGC, 17-388aa, Human, His tag, Baculovirus

Cat. Num. AAA206391

• Gene Names: PGC; PEPC; PGII
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: PGC; 17-388aa; Human; His tag; Baculovirus; Gastricsin; PEPC; PGII; PEPC_HUMAN; pepsin C; Pepsinogen C; pepsinogen group II; preprogastricsin; Progastricsin; PGC recombinant protein

• Host: Baculovirus
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: AVVKVPLKKF KSIRETMKEK GLLGEFLRTH KYDPAWKYRF GDLSVTYEPM AYMDAAYFGE ISIGTPPQNF LVLFDTGSSN LWVPSVYCQS QACTSHSRFN PSESSTYSTN GQTFSLQYGS GSLTGFFGYD TLTVQSIQVP NQEFGLSENE PGTNFVYAQF DGIMGLAYPA LSVDEATTAM QGMVQEGALT SPVFSVYLSN QQGSSGGAVV FGGVDSSLYT GQIYWAPVTQ ELYWQIGIEE FLIGGQASGW CSEGCQAIVD TGTSLLTVPQ QYMSALLQAT GAQEDEYGQF LVNCNSIQNL PSLTFIINGV EFPLPPSSYI LSNNGYCTVG VEPTYLSSQN GQPLWILGDV FLRSYYSVYD LGNNRVGFAT AALEHHHHHH
• Sequence Length: 388

• Applicable Applications for PGC recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PGC recombinant protein

PGC, also known as gastricsin, is an aspartic proteinase that belongs to the peptidase family A1. It is synthesized in the gastric mucosa as inactive precursors. It contains a prosegment that serves to stabilize the inactive form and prevent entry of the substrate to the active site. Serum levels of PGC are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis. Recombinant human PGC, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques

Product Categories/Family for PGC recombinant protein

Epigenomics (Transcription & Translation)

References

Richter C., et al. (1998) Biochem J. 335:481-490.; Westerveld BD., et al. (1987) Gastroenterology. 93:774-778.

NCBI and Uniprot Product Information

• NCBI GI #: 4505757
• NCBI GeneID: 5225
• NCBI Accession #: NP_002621
• NCBI GenBank Nucleotide #: NM_002630.3
• UniProt Accession #: P20142; Q5T3D7; Q5T3D8; B4DVZ3
• Molecular Weight: 41.6kDa (380aa) 40-57kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: gastricsin isoform 1 preproprotein
• NCBI Official Synonym Full Names: progastricsin
• NCBI Official Symbol: PGC
• NCBI Official Synonym Symbols: PEPC; PGII
• NCBI Protein Information: gastricsin
• UniProt Protein Name: Gastricsin
• Protein Family: PGC-1 and ERR-induced regulator in muscle protein
• UniProt Gene Name: PGC

NCBI Description

This gene encodes an aspartic proteinase that belongs to the peptidase family A1. The encoded protein is a digestive enzyme that is produced in the stomach and constitutes a major component of the gastric mucosa. This protein is also secreted into the serum. This protein is synthesized as an inactive zymogen that includes a highly basic prosegment. This enzyme is converted into its active mature form at low pH by sequential cleavage of the prosegment that is carried out by the enzyme itself. Polymorphisms in this gene are associated with susceptibility to gastric cancers. Serum levels of this enzyme are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 1. [provided by RefSeq, Oct 2009]

Uniprot Description

Hydrolyzes a variety of proteins.