Western Blot (WB)
(Anti-PGC rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:42 kDa Observed band size:42 kDa (We are unsure as to the identity of these extra bands.))
Rabbit anti-Human Pepsinogen C/PGC Polyclonal Antibody | anti-PGC antibody
Anti-Pepsinogen C/PGC Antibody, Rabbit Polyclonal
Gene Names
PGC; PEPC; PGII
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
Pepsinogen C/PGC; Polyclonal Antibody; Anti-Pepsinogen C/PGC Antibody; Rabbit Polyclonal; Pepsinogen C/PGC Antibody; Rabbit PAb; Antigen Affinity Purified; progastricsin (pepsinogen C); Anti-PEPC Antibody; Anti-PGII Antibody; anti-PGC antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human Pepsinogen C/PGC
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-PGC antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:2000
ELISA: 1:5000-1:10,000
IHC-P: 1:1000-1:10,000
ELISA: 1:5000-1:10,000
IHC-P: 1:1000-1:10,000
Immunogen
Recombinant Human Pepsinogen C/PGC Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human Pepsinogen C/PGC (rh Pepsinogen C/PGC). Pepsinogen C/PGC specific IgG was purified by Human Pepsinogen C/PGC affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-PGC rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:42 kDa Observed band size:42 kDa (We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-PGC rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:42 kDa Observed band size:42 kDa (We are unsure as to the identity of these extra bands.))
Immunohistochemistry (IHC)
(Immunochemical staining of human PGC in human stomach with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human PGC in human stomach with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-PGC antibody
Pepsinogen C, also known as PGC, is an aspartic proteinase that belongs to the peptidase family A1. Pepsinogen C is synthesized in the gastric mucosa as inactive precursors, known as zymogens. Pepsinogen C contains a prosegment that serves to stabilize the inactive form and prevent entry of the substrate to the active site. At low PH conditions, Pepsinogen C undergoes conversion into active enzyme. Pepsinogen C has been found expressed in all regions of the stomach mucosa and also in the proximal duodenal mucosa. In stomach cancer tissues and cancer cell lines, the expressions of the pepsinogen genes were decreased or lost, in good accordance with their pepsinogen productions. No gross structural changes of the pepsinogen genes were observed in these cancers, but the methylation patterns of the pepsinogen genes were found to be altered in different ways in different cancers. Serum levels of Pepsinogen C are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis.
References
Richter C, et al. (1998) Mechanism of activation of the gastric aspartic proteinases: pepsinogen, progastricsin and prochymosin. Biochem J. 1 (335): 481-90.Westerveld BD, et al. (1987) Gastric proteases in Barrett's esophagus. Gastroenterology. 93 (4): 774-8.Ichinose M, et al. (1991) Methylation and expression of human pepsinogen genes in normal tissues and their alteration in stomach cancer. Jpn J Cancer Res. 82 (6): 686-92.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34,208 Da
NCBI Official Full Name
gastricsin isoform 1 preproprotein
NCBI Official Synonym Full Names
progastricsin (pepsinogen C)
NCBI Official Symbol
PGC
NCBI Official Synonym Symbols
PEPC; PGII
NCBI Protein Information
gastricsin; pepsin C; pepsinogen C; preprogastricsin; pepsinogen group II
Protein Family
Similar Products
Product Notes
The PGC (Catalog #AAA8106144) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Pepsinogen C/PGC Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Pepsinogen C/PGC can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 IHC-P: 1:1000-1:10,000. Researchers should empirically determine the suitability of the PGC for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pepsinogen C/PGC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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