Rabbit anti-Human T Polyclonal Antibody | anti-T antibody

T, NT (T, Brachyury protein, Protein T) (AP)

Cat. Num. AAA6353127

• Gene Names: T; TFT; SAVA
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: T; Polyclonal Antibody; NT (T; Brachyury protein; Protein T) (AP); Protein T; anti-T antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human T.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-T antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 15-43 in the N-terminal region of human T, UniProt Accession #O15178. Species sequence homology: chicken, mouse
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in MDA-MB453 cell line lysates (35ug/lane) using 042546. This demonstrates that 042546 detected the T protein (arrow).)

Immunofluorescence (IF)

(Fluorescent confocal image of Hela cells stained with 042546. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with 042546 (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10ug/ml, 10 min). T immunoreactivity is localized to Nucleus significantly.)

Related Product Information for anti-T antibody

The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.

Product Categories/Family for anti-T antibody

Antibodies; Transcription Factors

References

1. Jambhekar, N.A., et al. Arch. Pathol. Lab. Med. 134(8):1181-1187(2010). 2. Docherty, S.J., et al. BMC Genet. 11:61 (2010). 3. Yang, X.R., et al. Nat. Genet. 41(11):1176-1178(2009). 4. Oakley, G.J., et al. Mod. Pathol. 21(12):1461-1469(2008). 5. Bondue, A., et al. Cell Stem Cell 3(1):69-84(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4507339
• NCBI GeneID: 6862
• NCBI Accession #: NP_003172.1
• NCBI GenBank Nucleotide #: NM_003181.3
• UniProt Accession #: O15178; Q4KMP4; E7ERD6
• Molecular Weight: 41,078 Da
• NCBI Official Full Name: brachyury protein isoform 1
• NCBI Official Synonym Full Names: T brachyury transcription factor
• NCBI Official Symbol: T
• NCBI Official Synonym Symbols: TFT; SAVA
• NCBI Protein Information: brachyury protein
• UniProt Protein Name: Brachyury protein
• Protein Family: THP3
• UniProt Gene Name: T
• UniProt Entry Name: BRAC_HUMAN

NCBI Description

The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

Brachyury: Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site. Genetic variations in T are associated with susceptibility to neural tube defects (NTD). NTD are common congenital malformations. Spina bifida, which results from malformations in the caudal region of the neural tube, is compatible with life but associated with significant morbidity, including lower limb paralysis. T is involved in susceptibility to the development of chordoma (CHDM). Chordomas are rare, clinically malignant tumors derived from notochordal remnants. They occur along the length of the spinal axis, predominantly in the sphenooccipital, vertebral and sacrococcygeal regions. They are characterized by slow growth, local destruction of bone, extension into adjacent soft tissues and rarely, distant metastatic spread. Susceptibility to development of chordomas is due to a T gene duplication.

Protein type: Motility/polarity/chemotaxis; DNA-binding; Cell cycle regulation; Transcription factor

Chromosomal Location of Human Ortholog: 6q27

Cellular Component: cytoplasm; nuclear chromatin; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; heart morphogenesis; somitogenesis; neural plate morphogenesis; embryonic skeletal development; mesoderm migration; notochord formation; negative regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; signal transduction; BMP signaling pathway; determination of anterior/posterior axis, embryo; positive regulation of cell proliferation; neural tube closure; penetration of zona pellucida; mesoderm development; vasculogenesis

Disease: Neural Tube Defects; Sacral Agenesis With Vertebral Anomalies