Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Cerebellum Tissue; Primary Ab: 10ug/ml Mouse Anti-Human SEMA5B AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
Mouse anti-Human Semaphorin 5B (SEMA5B) Monoclonal Antibody | anti-SEMA5B antibody
Monoclonal Antibody to Semaphorin 5B (SEMA5B)
Gene Names
SEMA5B; SemG; SEMAG
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Semaphorin 5B (SEMA5B); Monoclonal Antibody; Monoclonal Antibody to Semaphorin 5B (SEMA5B); SEMAG; SemG; Sema Domain; Immunoglobulin Domain (Ig); Transmembrane Domain (TM)and Short Cytoplasmic Domain 5B; anti-SEMA5B antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b Kappa
Clone Number
C11
Specificity
The antibody is a mouse monoclonal antibody raised against SEMA5B. It has been selected for its ability to recognize SEMA5B in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Liquid; PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
1mg/mL (varies by lot)
Sequence
Gly350~Pro602
Sequence Length
1057
Applicable Applications for anti-SEMA5B antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 0.2-2ug/mL; 1:500-5000
IHC: 5-20ug/mL; 1:50-200
ICC: 5-20ug/mL; 1:50-200
IHC: 5-20ug/mL; 1:50-200
ICC: 5-20ug/mL; 1:50-200
Conjugation
None
Cross Reactivity
Human
Preparation and Storage
Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Cerebellum Tissue; Primary Ab: 10ug/ml Mouse Anti-Human SEMA5B AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Cerebellum Tissue; Primary Ab: 10ug/ml Mouse Anti-Human SEMA5B AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
semaphorin-5B isoform 3
NCBI Official Synonym Full Names
semaphorin 5B
NCBI Official Symbol
SEMA5B
NCBI Official Synonym Symbols
SemG; SEMAG
NCBI Protein Information
semaphorin-5B
UniProt Protein Name
Semaphorin-5B
Protein Family
UniProt Gene Name
SEMA5B
UniProt Synonym Gene Names
KIAA1445; SEMAG
NCBI Description
This gene encodes a member of the semaphorin protein family which regulates axon growth during development of the nervous system. The encoded protein has a characteristic Sema domain near the N-terminus, through which semaphorins bind to plexin, and five thrombospondin type 1 repeats in the C-terminal region of the protein. The protein product may be cleaved and exist as a secreted molecule (PMID: 19463192). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
Uniprot Description
May act as positive axonal guidance cues.
Research Articles on SEMA5B
1. SEMA5B could possibly serve as a candidate gene for alterations associated with asbestos exposure.
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Product Notes
The SEMA5B sema5b (Catalog #AAA2111331) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Semaphorin 5B (SEMA5B) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Semaphorin 5B (SEMA5B) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 0.2-2ug/mL; 1:500-5000 IHC: 5-20ug/mL; 1:50-200 ICC: 5-20ug/mL; 1:50-200. Researchers should empirically determine the suitability of the SEMA5B sema5b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Gly350~Pro 602. It is sometimes possible for the material contained within the vial of "Semaphorin 5B (SEMA5B), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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