TNFRSF18 recombinant protein

TNFRSF18, 26-162aa, Human, hIgG-His tag, Baculovirus

Cat. Num. AAA206336

• Gene Names: TNFRSF18; AITR; GITR; CD357; GITR-D
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: TNFRSF18; 26-162aa; Human; hIgG-His tag; Baculovirus; Tumor necrosis factor receptor superfamily member 18 isoform 1; AITR; CD357; GITR; GITR-D; TNFRSF18 recombinant protein

• Host: Baculovirus
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: QRPTGGPGCG PGRLLLGTGT DARCCRVHTT RCCRDYPGEE CCSEWDCMCV QPEFHCGDPC CTTCRHHPCP PGQGVQSQGK FSFGFQCIDC ASGTFSGGHE GHCKPWTDCT QFGFLTVFPG NKTHNAVCVP GSPPAEPLEP KSCDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSRDE LTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK HHHHHH
• Sequence Length: 241

• Applicable Applications for TNFRSF18 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: hIgG-His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for TNFRSF18 recombinant protein

TNFRSF18, also known as tumor necrosis factor receptor superfamily member 18 isoform 1, is receptor for TNFSF18. It seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. TNFRSF18 mediated NF-kappa-B activation via the TRAF2/NIK pathway. Also, this protein reciprocally stimulated and activate intracellular signals regulating immune functions. In particular, GITR-driven T-cell co-stimulation was found to be the main mechanism by which the GITRL-GITR system contributes to tumor rejection and the development of autoimmune/inflammatory diseases. Recombinant human TNFRSF18, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for TNFRSF18 recombinant protein

Hormones & Cytokines; Immunology

References

Lacal PM., et al. (2013) J. Pharmacol. Exp. Ther. 347:164-172.; Xufre C., et al. (2013) Int. Immunol. 25:563-574.; Shimizu J., et al. (2002) Nat. Immunol. 3:135-142.

NCBI and Uniprot Product Information

• NCBI GI #: 4759246
• NCBI GeneID: 8784
• NCBI Accession #: NP_004186
• NCBI GenBank Nucleotide #: NM_004195.2
• UniProt Accession #: Q9Y5U5; O95851; Q5U0I4; Q9NYJ9; B1AME1
• Molecular Weight: 41.6kDa (376aa) 40-57KDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 18 isoform 1
• NCBI Official Synonym Full Names: TNF receptor superfamily member 18
• NCBI Official Symbol: TNFRSF18
• NCBI Official Synonym Symbols: AITR; GITR; CD357; GITR-D
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 18
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 18
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: TNFRSF18
• UniProt Synonym Gene Names: AITR; GITR

NCBI Description

This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Feb 2011]

Uniprot Description

Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.