Human NF2 (Neurofibromin 2) ELISA Kit | NF2 elisa kit

Human NF2 (Neurofibromin 2) ELISA Kit

Cat. Num. AAA7606241

• Gene Names: NF2; ACN; SCH; BANF
• Reactivity: Human
• Synonyms: NF2 (Neurofibromin 2); Human NF2 (Neurofibromin 2) ELISA Kit; NF2 elisa kit

• Reactivity: Human
• Sequence Length: 533

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NF2 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- NF2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-NF2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the NF2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of NF2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 119580237
• NCBI GeneID: 4771
• Molecular Weight: 24,515 Da
• NCBI Official Full Name: neurofibromin 2 (bilateral acoustic neuroma), isoform CRA_l
• NCBI Official Synonym Full Names: neurofibromin 2
• NCBI Official Symbol: NF2
• NCBI Official Synonym Symbols: ACN; SCH; BANF
• NCBI Protein Information: merlin
• UniProt Protein Name: Merlin
• Protein Family: Merlin
• UniProt Gene Name: NF2
• UniProt Synonym Gene Names: SCH

NCBI Description

This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This gene product has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts. [provided by RefSeq, Jul 2008]

Uniprot Description

Merlin: a moesin-ezrin-radizin-like protein. Interacts with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. Expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations are associated with neurofibromatosis type II. Two predominant isoforms and eight minor isoforms are produced by alternatively spliced transcripts.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis; Tumor suppressor

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: cytoplasm; cytoskeleton; cytosol; early endosome; membrane; nucleolus; nucleus; perinuclear region of cytoplasm; plasma membrane

Molecular Function: protein binding

Biological Process: actin cytoskeleton organization and biogenesis; negative regulation of cell migration; negative regulation of cell proliferation; negative regulation of cell-cell adhesion; negative regulation of cell-matrix adhesion; negative regulation of DNA replication; negative regulation of JAK-STAT cascade; positive regulation of stress fiber formation; Schwann cell proliferation

Disease: Meningioma, Familial, Susceptibility To; Neurofibromatosis, Type Ii; Schwannomatosis 1