POLL recombinant protein

POLL, 1-300aa, Human, His tag, E Coli

Cat. Num. AAA204443

• Gene Names: POLL; BETAN; POLKAPPA
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: POLL; 1-300aa; Human; His tag; E Coli; DNA polymerase lambda; BETA-N; POL-KAPPA; POLL recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MLMHHQKYLQ RFLGGKREKK QKEACSIPGI GKRMAEKIIE ILESGHLRKL DHISESVPVL ELFSNIWGAG TKTAQMWYQQ GFRSLEDIRS QASLTTQQAI GLKHYSDFLE RMPREEATEI EQTVQKAAQA FNSGLLCVAC GSYRRGKATC GDVDVLITHP DGRSHRGIFS RLLDSLRQEG FLTDDLVSQE ENGQQQKYLG VCRLPGPGRR HRRLDIIVVP YSEFACALLY FTGSAHFNRS MRALAKTKGM SLSEHALSTA VVRNTHGCKV GPGRVLPTPT EKDVFRLLGL PYREPAERDW
• Sequence Length: 300

• Applicable Applications for POLL recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for POLL recombinant protein

DNA polymerase lambda, also known as POLL, is a DNA polymerase involved in base excision repair (BER). POLL is a low-fidelity polymerase which plays a role in both spontaneous and DNA damage-induced mutagenesis. In addition, POLL may play a role in the mutagenic bypass of T-T dimers. Pol lambda possesses terminal transferase and 5'-deoxyribose-5-phosphate lyase activity. These activities have been found to be coordinated by PCNA and replication protein A. Recombinant human POLL protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for POLL recombinant protein

Enzymes & Proteases

References

O Wang J., et al. (2001) Cancer Res. 61:5366-5369.; Zhang Y., et al. (2000) Nucleic Acids Res. 28:4147-4156.

NCBI and Uniprot Product Information

• NCBI GI #: 113812195
• NCBI GeneID: 27343
• Molecular Weight: 36 kDa (320aa), confirmed by MALDI-TOF
• NCBI Official Full Name: POLL protein
• NCBI Official Synonym Full Names: DNA polymerase lambda
• NCBI Official Symbol: POLL
• NCBI Official Synonym Symbols: BETAN; POLKAPPA
• NCBI Protein Information: DNA polymerase lambda
• UniProt Protein Name: DNA polymerase lambda
• Protein Family: Pollen allergen
• UniProt Gene Name: POLL
• UniProt Synonym Gene Names: Pol beta2

NCBI Description

This gene encodes a DNA polymerase. DNA polymerases catalyze DNA-template-directed extension of the 3'-end of a DNA strand. This particular polymerase, which is a member of the X family of DNA polymerases, likely plays a role in non-homologous end joining and other DNA repair processes. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2010]

Uniprot Description

DNA polymerase that functions in several pathways of DNA repair (PubMed:11457865, PubMed:19806195, PubMed:20693240). Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA (PubMed:11457865, PubMed:19806195). Also contributes to DNA double-strand break repair by non-homologous end joining and homologous recombination (PubMed:19806195, PubMed:20693240). Has both template-dependent and template-independent (terminal transferase) DNA polymerase activities (PubMed:10982892, PubMed:10887191, PubMed:12809503, PubMed:14627824, PubMed:15537631, PubMed:19806195). Has also a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity (PubMed:11457865, PubMed:19806195).