ADH1A recombinant protein

ADH1A, 1-375aa, Human, His tag, E Coli

Cat. Num. AAA204173

• Gene Names: ADH1A; ADH1
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: ADH1A; 1-375aa; Human; His tag; E Coli; Alcohol dehydrogenase 1A; ADH1; ADH1A recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MSTAGKVIKC KAAVLWELKK PFSIEEVEVA PPKAHEVRIK MVAVGICGTD DHVVSGTMVT PLPVILGHEA AGIVESVGEG VTTVKPGDKV IPLAIPQCGK CRICKNPESN YCLKNDVSNP QGTLQDGTSR FTCRRKPIHH FLGISTFSQY TVVDENAVAK IDAASPLEKV CLIGCGFSTG YGSAVNVAKV TPGSTCAVFG LGGVGLSAIM GCKAAGAARI IAVDINKDKF AKAKELGATE CINPQDYKKP IQEVLKEMTD GGVDFSFEVI GRLDTMMASL LCCHEACGTS VIVGVPPDSQ NLSMNPMLLL TGRTWKGAIL GGFKSKECVP KLVADFMAKK FSLDALITHV LPFEKINEGF DLLHSGKSIR TILMF
• Sequence Length: 375

• Applicable Applications for ADH1A recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for ADH1A recombinant protein

Alcohol dehydrogenase 1A, also known as ADH1A, belongs to the alcohol dehydrogenase family. ADH1 is a monomorphic and predominant in fetal and infant livers, becoming less active in gestation and only weakly active during adulthood. ADH1A plays a major role in ethanol metabolism. With the coenzyme NAD, ADH catalyzes the reversible conversion of organic alcohols to ketones or aldehydes. The physiologic function for ADH1A in the liver is the removal of ethanol formed by microorganisms in the intestinal tract. Recombinant human ADH1A protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for ADH1A recombinant protein

Metabolism

References

Jelski W., et al. (2007) Dig Dis Sci. 52:1513-1516.; Smith M., et al. (1973) Ann Hum Genet. 36:401-414.;

NCBI and Uniprot Product Information

• NCBI GI #: 4501929
• NCBI GeneID: 124
• NCBI Accession #: NP_000658
• NCBI GenBank Nucleotide #: NM_000667.3
• UniProt Accession #: P07327; Q17R68; A8K3E3
• Molecular Weight: 42 kDa (395aa) confirmed by MALDI-TOF
• NCBI Official Full Name: alcohol dehydrogenase 1A
• NCBI Official Synonym Full Names: alcohol dehydrogenase 1A (class I), alpha polypeptide
• NCBI Official Symbol: ADH1A
• NCBI Official Synonym Symbols: ADH1
• NCBI Protein Information: alcohol dehydrogenase 1A
• UniProt Protein Name: Alcohol dehydrogenase 1A
• UniProt Gene Name: ADH1A
• UniProt Synonym Gene Names: ADH1

NCBI Description

This gene encodes a member of the alcohol dehydrogenase family. The encoded protein is the alpha subunit of class I alcohol dehydrogenase, which consists of several homo- and heterodimers of alpha, beta and gamma subunits. Alcohol dehydrogenases catalyze the oxidation of alcohols to aldehydes. This gene is active in the liver in early fetal life but only weakly active in adult liver. This gene is found in a cluster with six additional alcohol dehydrogenase genes, including those encoding the beta and gamma subunits, on the long arm of chromosome 4. Mutations in this gene may contribute to variation in certain personality traits and substance dependence. [provided by RefSeq, Nov 2010]

Uniprot Description

MiscellaneousThere are 7 different ADH's isozymes in human: three belongs to class-I: alpha, beta, and gamma, one to class-II: pi, one to class-III: chi, one to class-IV: ADH7 and one to class-V: ADH6.