uGDH active protein
uGDH, 1-494aa, Human, His tag, E Coli (Bioactivity Validated)
Gene Names
UGDH; GDH; UGD; UDPGDH; UDP-GlcDH
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
uGDH; 1-494aa; Human; His tag; E Coli (Bioactivity Validated); uDP-glucose dehydrogenase; GDH; uDP-GlcDH; uDPGDH; uGD; uDP-glucose 6-dehydrogenase; uGDH active protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 1mM DTT, 0.1M NaCl, and 1mM EDTA.
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELEM FEIKKICCIG AGYVGGPTCS VIAHMCPEIR VTVVDVNESR INAWNSPTLP IYEPGLKEVV ESCRGKNLFF STNIDDAIKE ADLVFISVNT PTKTYGMGKG RAADLKYIEA CARRIVQNSN GYKIVTEKST VPVRAAESIR RIFDANTKPN LNLQVLSNPE FLAEGTAIKD LKNPDRVLIG GDETPEGQRA VQALCAVYEH WVPREKILTT NTWSSELSKL AANAFLAQRI SSINSISALC EATGADVEEV ATAIGMDQRI GNKFLKASVG FGGSCFQKDV LNLVYLCEAL NLPEVARYWQ QVIDMNDYQR RRFASRIIDS LFNTVTDKKI AILGFAFKKD TGDTRESSSI YISKYLMDEG AHLHIYDPKV PREQIVVDLS HPGVSEDDQV SRLVTISKDP YEACDGAHAV VICTEWDMFK ELDYERIHKK MLKPAFIFDG RRVLDGLHNE LQTIGFQIET IGKKVSSKRI PYAPSGEIPK FSLQDPPNKK PKV
Sequence Length
494
Applicable Applications for uGDH active protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Biological Activity
Specific activity is > 2500 pmol/min/ug, and is defined as the amount of enzyme that convert 1.0pmole of UDP-glucose to UDP-glucuronate per minute at pH 8.7 at 37C.
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
Related Product Information for uGDH active protein
uGDH is a member of the uDP-glucose/GDP-mannose dehydrogenase family and is a ubiquitously expressed protein most abundant in the liver. This protein converts uDP-glucose to uDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis. Recombinant human uGDH protein, fused to his-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques
Product Categories/Family for uGDH active protein
References
Bontemps Y., et al. (2003) J Biol Chem. 278 (24):21566-75. ; Vatsyayan J., et al. (2006) Biosci Biotechnol Biochem. 70 (2):401-10. ;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59.5 kDa (533aa) confirmed by MALDI-TOF
NCBI Official Full Name
UDP-glucose 6-dehydrogenase isoform 1
NCBI Official Synonym Full Names
UDP-glucose 6-dehydrogenase
NCBI Official Symbol
UGDH
NCBI Official Synonym Symbols
GDH; UGD; UDPGDH; UDP-GlcDH
NCBI Protein Information
UDP-glucose 6-dehydrogenase
UniProt Protein Name
UDP-glucose 6-dehydrogenase
UniProt Gene Name
UGDH
UniProt Synonym Gene Names
UDP-Glc dehydrogenase; UDP-GlcDH; UDPGDH
NCBI Description
The protein encoded by this gene converts UDP-glucose to UDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis. The expression of this gene is up-regulated by transforming growth factor beta and down-regulated by hypoxia. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
Uniprot Description
Involved in the biosynthesis of glycosaminoglycans; hyaluronan, chondroitin sulfate, and heparan sulfate.
