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Western Blot (WB) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UGDH antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: NIH-3T3 cell lysate Lane 3.: HepG2 cell lysate Lane 4.: A549 cell lysate Lane 5.: MCF7 cell lysate )

Mouse anti-Human UGDH Monoclonal Antibody | anti-UGDH antibody

UGDH antibody

Gene Names
UGDH; GDH; UGD; UDPGDH; UDP-GlcDH
Reactivity
Human
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence
Purity
By protein-A affinity chromatography
Synonyms
UGDH; Monoclonal Antibody; UGDH antibody; uDP-glucose dehydrogenase; GDH; uDP-GlcDH; uDPGDH; uGD; uDP-glucose 6-dehydrogenase; anti-UGDH antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT2G11
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
494
Applicable Applications for anti-UGDH antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Antigen Species
Human
Immunogen
Recombinant human UGDH (1-494aa) purified from E. coli
Clone Note
Anti-human UGDH mAb, clone AT2G11, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human UGDH protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.

Western Blot (WB)

(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UGDH antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: NIH-3T3 cell lysate Lane 3.: HepG2 cell lysate Lane 4.: A549 cell lysate Lane 5.: MCF7 cell lysate )

Western Blot (WB) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UGDH antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: NIH-3T3 cell lysate Lane 3.: HepG2 cell lysate Lane 4.: A549 cell lysate Lane 5.: MCF7 cell lysate )

Immunofluorescence (IF)

(ICC/IF analysis of UGDH in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human UGDH antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)

Immunofluorescence (IF) (ICC/IF analysis of UGDH in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human UGDH antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Related Product Information for anti-UGDH antibody
UGDH is a member of the UDP-glucose/GDP-mannose dehydrogenase family and is a ubiquitously expressed protein most abundant in the liver. This protein converts UDP-glucose to UDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis.
Product Categories/Family for anti-UGDH antibody
References
Bontemps Y., et al. (2003) J Biol Chem. 278 (24): 21566-75.; Vatsyayan J., et al. (2006) Biosci Biotechnol Biochem. 70 (2): 401-10.; Egger S., et al. (2011) J Biol Chem. 286 (27): 23877-87.;

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
44,415 Da
NCBI Official Full Name
UDP-glucose 6-dehydrogenase isoform 1
NCBI Official Synonym Full Names
UDP-glucose 6-dehydrogenase
NCBI Official Symbol
UGDH
NCBI Official Synonym Symbols
GDH; UGD; UDPGDH; UDP-GlcDH
NCBI Protein Information
UDP-glucose 6-dehydrogenase
UniProt Protein Name
UDP-glucose 6-dehydrogenase
UniProt Gene Name
UGDH
UniProt Synonym Gene Names
UDP-Glc dehydrogenase; UDP-GlcDH; UDPGDH

NCBI Description

The protein encoded by this gene converts UDP-glucose to UDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis. The expression of this gene is up-regulated by transforming growth factor beta and down-regulated by hypoxia. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Uniprot Description

Involved in the biosynthesis of glycosaminoglycans; hyaluronan, chondroitin sulfate, and heparan sulfate.

Research Articles on UGDH

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Product Notes

The UGDH ugdh (Catalog #AAA200424) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The UGDH antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's UGDH can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the UGDH ugdh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UGDH, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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