Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Human Arachidonate-12-Lipoxygenase Polyclonal Antibody | anti-ALOX12B antibody
Polyclonal Antibody to Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B)
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded prostate gland tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded prostate gland tissue))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an enzyme involved in the conversion of arachidonic acid to 12R-hydroxyeicosatetraenoic acid. Mutations in this gene are associated with nonbullous congenital ichthyosiform erythroderma. [provided by RefSeq, Sep 2015]
Uniprot Description
ALOX12B: Converts arachidonic acid to 12R- hydroperoxyeicosatetraenoic acid (12R-HPETE). Defects in ALOX12B are a cause of non-bullous congenital ichthyosiform erythroderma (NCIE). NCIE is a non- bullous ichthyosis, a skin disorder characterized by abnormal cornification of the epidermis. Most affected individuals are born with a tight, shiny, translucent covering called collodion membrane. The collodion membrane subsequently evolves into generalized scaling and intense redness of the skin. Clinical features are milder than in lamellar ichthyoses and demonstrate a greater variability in the intensity of erythema, size and type of scales. In contrast to lamellar ichthyoses, scales are usually white, fine and powdery, and palms and soles are severely affected. Patients suffer from palmoplantar keratoderma, often with painful fissures, digital contractures, and loss of pulp volume. Belongs to the lipoxygenase family.
Protein type: EC 1.13.11.-; Lipid Metabolism - arachidonic acid; Oxidoreductase
Chromosomal Location of Human Ortholog: 17p13.1
Cellular Component: cytosol
Molecular Function: arachidonate 12-lipoxygenase activity; iron ion binding; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; protein binding
Biological Process: arachidonic acid metabolic process; ceramide biosynthetic process; hepoxilin biosynthetic process; linoleic acid metabolic process; lipoxygenase pathway; positive regulation of MAPK cascade; protein lipidation; sphingolipid metabolic process
Disease: Ichthyosis, Congenital, Autosomal Recessive 2
Research Articles on ALOX12B
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Product Notes
The ALOX12B alox12b (Catalog #AAA2033929) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Arachidonate-12-Lipoxygenase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-1:5000. Researchers should empirically determine the suitability of the ALOX12B alox12b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Arachidonate-12-Lipoxygenase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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