Rabbit anti-Human MYO6 Polyclonal Antibody | anti-MYO6 antibody

MYO6, CT (R1181) (MYO6, KIAA0389, Unconventional myosin-VI, Unconventional myosin-6) (Biotin)

Cat. Num. AAA6323464

• Gene Names: MYO6; DFNA22; DFNB37
• Reactivity: Human
• Applications: Immunofluorescence, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MYO6; Polyclonal Antibody; CT (R1181) (MYO6; KIAA0389; Unconventional myosin-VI; Unconventional myosin-6) (Biotin); Unconventional myosin-6; anti-MYO6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-MYO6 antibody: Immunofluorescence (IF), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 1:10-50; ELISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: MYO6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1173-1203 amino acids from the C-terminal region of human MYO6.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MYO6 Antibody (C-term R1181) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the MYO6 antibody detected the MYO6 protein (arrow).)

Immunohistochemistry (IHC)

(MYO6 Antibody (C-term R1181) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MYO6 Antibody (C-term R1181) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of MYO6 Antibody (C-term R1181) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-MYO6 antibody

This gene encodes a protein involved intracellular vesicle and organelle transport, especially in the hair cell of the inner ear. Mutations in this gene have been found in patients with non-syndromic autosomal dominant and recessive hearing loss.

Product Categories/Family for anti-MYO6 antibody

Antibodies; KIAA; orf

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Nishikawa, S., et al. Cell 142(6):879-888(2010). Cho, S.J., et al. J. Biol. Chem. 285(35):27159-27166(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Szczyrba, J., et al. Mol. Cancer Res. 8(4):529-538(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 92859701
• NCBI GeneID: 4646
• NCBI Accession #: NP_004990.3
• NCBI GenBank Nucleotide #: NM_004999.3
• UniProt Accession #: Q9UM54; Q5TEM5; Q5TEM6; Q5TEM7; Q9BZZ7; Q9UEG2; A6H8V4; E1P540
• Molecular Weight: 149,691 Da
• NCBI Official Full Name: unconventional myosin-VI isoform 1
• NCBI Official Synonym Full Names: myosin VI
• NCBI Official Symbol: MYO6
• NCBI Official Synonym Symbols: DFNA22; DFNB37
• NCBI Protein Information: unconventional myosin-VI; unconventional myosin-6
• UniProt Protein Name: Unconventional myosin-VI
• Protein Family: Unconventional myosin
• UniProt Gene Name: MYO6
• UniProt Synonym Gene Names: KIAA0389
• UniProt Entry Name: MYO6_HUMAN

Uniprot Description

MYO6: a molecular motor involved in intracellular vesicle and organelle transport. It is one of the so-called unconventional myosins.

Protein type: Motility/polarity/chemotaxis; Actin-binding; Motor

Chromosomal Location of Human Ortholog: 6q13

Cellular Component: Golgi apparatus; nuclear membrane; microvillus; lysosomal membrane; cytoplasmic membrane-bound vesicle; clathrin coated vesicle membrane; coated pit; DNA-directed RNA polymerase II, holoenzyme; cell cortex; clathrin-coated endocytic vesicle; cytosol; nucleoplasm; filamentous actin; ruffle; cell soma; membrane; perinuclear region of cytoplasm; endocytic vesicle; apical part of cell; axon; cytoplasm; unconventional myosin complex; plasma membrane; nucleus

Molecular Function: actin filament binding; calmodulin binding; minus-end directed microfilament motor activity; protein binding; motor activity; actin binding; ADP binding; ATP binding

Biological Process: response to drug; inner ear morphogenesis; DNA damage response, signal transduction by p53 class mediator; metabolic process; actin filament-based movement; regulation of synaptic plasticity; auditory receptor cell differentiation; regulation of secretion; locomotory behavior; endocytosis; synaptic transmission; intracellular protein transport; synaptogenesis; sensory perception of sound; glutamate secretion; dendrite development; positive regulation of transcription from RNA polymerase II promoter; protein targeting

Disease: Deafness, Autosomal Recessive 37; Deafness, Autosomal Dominant 22