GATA Binding Protein 2 Recombinant Protein | GATA2 recombinant protein

Recombinant GATA Binding Protein 2 (GATA2)

Cat. Num. AAA2031734

• Gene Names: GATA2; DCML; IMD21; NFE1B; MONOMAC
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: GATA Binding Protein 2; Recombinant GATA Binding Protein 2 (GATA2); GATA2 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 9.6 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-TVSP FSKTPLHPSA AGAPGGPLSV YPGAAGGSGG GSGSSVASLT PTAAHSGSHL FGFPPTPPKE VSPDPSTTGA ASPASSSAGG SVARGEDKDG VKYQVSLSES MKMEGGSPLR PGLATMGTQP ATHHPIPTYP SYVPAAAHDY GSSLFHPGGF LGGPASSFTP KQRSKARSCS EGRECVNCGATATPLWRRDG TGHYLCNACG LYHKMNGQNR PLIKPKRR
• Sequence Length: 480

• Applicable Applications for GATA2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 26.3kDa
• Accurate Molecular Mass (KD): 30kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Mus musculus (Mouse)
• Fragment: Thr117~Arg338
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 30410997
• NCBI GeneID: 2624
• Molecular Weight: 49,106 Da
• NCBI Official Full Name: GATA binding protein 2
• NCBI Official Synonym Full Names: GATA binding protein 2
• NCBI Official Symbol: GATA2
• NCBI Official Synonym Symbols: DCML; IMD21; NFE1B; MONOMAC
• NCBI Protein Information: endothelial transcription factor GATA-2
• UniProt Protein Name: Endothelial transcription factor GATA-2
• Protein Family: GATA-binding factor
• UniProt Gene Name: GATA2
• UniProt Entry Name: GATA2_HUMAN

NCBI Description

This gene encodes a member of the GATA family of zinc-finger transcription factors that are named for the consensus nucleotide sequence they bind in the promoter regions of target genes. The encoded protein plays an essential role in regulating transcription of genes involved in the development and proliferation of hematopoietic and endocrine cell lineages. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]

Uniprot Description

GATA2: Transcriptional activator which regulates endothelin-1 gene expression in endothelial cells. Binds to the consensus sequence 5'-AGATAG-3'. Endothelial cells. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 3q21.3

Cellular Component: nucleoplasm; protein complex

Molecular Function: protein binding; zinc ion binding; chromatin binding; transcription factor activity; transcription factor binding

Biological Process: embryonic placenta development; inner ear morphogenesis; transcription, DNA-dependent; somatic stem cell maintenance; positive regulation of erythrocyte differentiation; cell maturation; regulation of histone acetylation; negative regulation of transcription from RNA polymerase II promoter; cell fate determination; negative regulation of fat cell differentiation; phagocytosis; cell differentiation in hindbrain; positive regulation of angiogenesis; negative regulation of macrophage differentiation; homeostasis of number of cells within a tissue; pituitary gland development; ventral spinal cord interneuron differentiation; commitment of a neuronal cell to a specific type of neuron in the forebrain; positive regulation of phagocytosis; negative regulation of Notch signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of megakaryocyte differentiation; blood coagulation; urogenital system development; central nervous system neuron development

Disease: Lymphedema, Primary, With Myelodysplasia; Immunodeficiency 21; Myelodysplastic Syndrome