Dual Specificity Phosphatase 5 Recombinant Protein | DUSP5 recombinant protein

Recombinant Dual Specificity Phosphatase 5 (DUSP5)

Cat. Num. AAA2031245

• Gene Names: DUSP5; DUSP; HVH3
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Dual Specificity Phosphatase 5; Recombinant Dual Specificity Phosphatase 5 (DUSP5); DUSP5 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MKVTSLDGRQ LRKMLRKEAA ARCVVLDCRP YLAFAASNVR GSLNVNLNSVVLRRARGGAV SARYVLPDEA ARARLLQEGG GGVAAVVVLD QGSRHWQKLREESAARVVLT SLLACLPAGP RVYFLKGGYE TFYSE
• Sequence Length: 384

• Applicable Applications for DUSP5 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 18.4kDa
• Accurate Molecular Mass (KD): 18kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Met1~Glu135
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 62865890
• NCBI GeneID: 1847
• NCBI Accession #: NP_004410.3
• NCBI GenBank Nucleotide #: NM_004419.3
• Molecular Weight: 42,047 Da
• NCBI Official Full Name: dual specificity protein phosphatase 5
• NCBI Official Synonym Full Names: dual specificity phosphatase 5
• NCBI Official Symbol: DUSP5
• NCBI Official Synonym Symbols: DUSP; HVH3
• NCBI Protein Information: dual specificity protein phosphatase 5
• UniProt Protein Name: Dual specificity protein phosphatase 5
• Protein Family: Dual specificity protein phosphatase
• UniProt Gene Name: DUSP5
• UniProt Synonym Gene Names: VH3
• UniProt Entry Name: DUS5_HUMAN

NCBI Description

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, is expressed in a variety of tissues with the highest levels in pancreas and brain, and is localized in the nucleus. [provided by RefSeq, Jul 2008]

Uniprot Description

DUSP5: a dual-specificity protein phosphatase containing one Rhodanese domain. Is a direct transcriptional target of p53. Targets include phosphorylated MAP kinases. Overexpression suppresses the growth of several types of human cancer cells. Induced by IL-2, IL-7, and IL-15 but not IL-4 in T cells.

Protein type: EC 3.1.3.48; EC 3.1.3.16; Protein phosphatase, dual-specificity; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10q25

Cellular Component: nucleus

Molecular Function: protein binding; protein tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity; MAP kinase tyrosine/serine/threonine phosphatase activity

Biological Process: endoderm formation; protein amino acid dephosphorylation; inactivation of MAPK activity