Mouse Dual Specificity Phosphatase 5 (DUSP5) ELISA Kit | DUSP5 elisa kit

Mouse Dual Specificity Phosphatase 5 (DUSP5) ELISA Kit

Cat. Num. AAA2705974

• Gene Names: DUSP5; DUSP; HVH3
• Reactivity: Mouse
• Synonyms: Dual Specificity Phosphatase 5 (DUSP5); Mouse Dual Specificity Phosphatase 5 (DUSP5) ELISA Kit; DUSP; HVH3; Dual specificity protein phosphatase hVH3; DUSP5 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Dual Specificity Phosphatase 5 (DUSP5).; No significant cross-reactivity or interference between Dual Specificity Phosphatase 5 (DUSP5) and analogues was observed.
• Sequence Length: 384

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dual Specificity Phosphatase 5 (DUSP5) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dual Specificity Phosphatase 5 (DUSP5) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DUSP5 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dual Specificity Phosphatase 5 (DUSP5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dual Specificity Phosphatase 5 (DUSP5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dual Specificity Phosphatase 5 (DUSP5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dual Specificity Phosphatase 5 (DUSP5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 62865890
• NCBI GeneID: 1847
• NCBI Accession #: NP_004410.3
• NCBI GenBank Nucleotide #: NM_004419.3
• UniProt Accession #: Q16690; Q12997; Q5T603
• Molecular Weight: 42,047 Da
• NCBI Official Full Name: dual specificity protein phosphatase 5
• NCBI Official Synonym Full Names: dual specificity phosphatase 5
• NCBI Official Symbol: DUSP5
• NCBI Official Synonym Symbols: DUSP; HVH3
• NCBI Protein Information: dual specificity protein phosphatase 5
• UniProt Protein Name: Dual specificity protein phosphatase 5
• Protein Family: Dual specificity protein phosphatase
• UniProt Gene Name: DUSP5
• UniProt Synonym Gene Names: VH3

NCBI Description

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, is expressed in a variety of tissues with the highest levels in pancreas and brain, and is localized in the nucleus. [provided by RefSeq, Jul 2008]

Uniprot Description

Dual specificity protein phosphatase; active with phosphotyrosine, phosphoserine and phosphothreonine residues. The highest relative activity is toward ERK1.