Carboxypeptidase A4 Recombinant Protein | CPA4 recombinant protein

Recombinant Carboxypeptidase A4 (CPA4)

Cat. Num. AAA2030189

• Gene Names: CPA4; CPA3
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Carboxypeptidase A4; Recombinant Carboxypeptidase A4 (CPA4); CPA4 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; KSPSSF NRPVDVLVPS VSLQAFKSFL RSQGLEYAVT IEDLQALLDNEDDEMQHNEG QERSSNNFNY GAYHSLEAIY HEMDNIAADF PDLARRVKIGHSFENRPMYV LKFSTGKGVR RPAVWLNAGI HSREWISQAT AIWTARKIVSDYQRDPAITS ILEKMDIFLL PVANPDGYVY TQTQNRLWRK TRSRNPGSSCIGADPNRNWN ASFAGKGASD NPCSEVYHGP HANSEVEVKS VVDFIQKHGNFKGFIDLHSY SQLLMYPYGY SVKKAPDAEE LDKVARLAAK ALASVSGTEYQVGPTCTTVY PASGSSIDWA YDNGIKFAFT FELRDTGTYG FLLPANQIIPTAEETWLGLK TIMEHVRDNL Y
• Sequence Length: 421

• Applicable Applications for CPA4 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 44.9kDa
• Accurate Molecular Mass (KD): 45kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Lys55~Tyr421
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 61743916
• NCBI GeneID: 51200
• NCBI Accession #: NP_057436.2
• NCBI GenBank Nucleotide #: NM_016352.3
• Molecular Weight: 43,570 Da
• NCBI Official Full Name: carboxypeptidase A4 preproprotein
• NCBI Official Synonym Full Names: carboxypeptidase A4
• NCBI Official Symbol: CPA4
• NCBI Official Synonym Symbols: CPA3
• NCBI Protein Information: carboxypeptidase A4
• UniProt Protein Name: Carboxypeptidase A4
• Protein Family: Carboxypeptidase
• UniProt Gene Name: CPA4
• UniProt Synonym Gene Names: CPA3
• UniProt Entry Name: CBPA4_HUMAN

NCBI Description

This gene is a member of the carboxypeptidase A/B subfamily, and it is located in a cluster with three other family members on chromosome 7. Carboxypeptidases are zinc-containing exopeptidases that catalyze the release of carboxy-terminal amino acids, and are synthesized as zymogens that are activated by proteolytic cleavage. This gene could be involved in the histone hyperacetylation pathway. It is imprinted and may be a strong candidate gene for prostate cancer aggressiveness. [provided by RefSeq, Jul 2008]

Uniprot Description

CPA4: Metalloprotease that could be involved in the histone hyperacetylation pathway. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; EC 3.4.17.-; Protease; Secreted

Chromosomal Location of Human Ortholog: 7q32

Cellular Component: extracellular region

Molecular Function: zinc ion binding; metallocarboxypeptidase activity

Biological Process: histone acetylation; proteolysis