Western Blot (WB)
(Sample: Recombinant PPARa, Mouse.)
Rabbit anti-Mouse Peroxisome Proliferator Activated Receptor Alpha Polyclonal Antibody | anti-PPARa antibody
Polyclonal Antibody to Peroxisome Proliferator Activated Receptor Alpha (PPARa)
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 24 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Sample: Recombinant PPARa, Mouse.)
Western Blot (WB)
(Sample: Recombinant PPARa, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue.)
NCBI and Uniprot Product Information
Uniprot Description
PPAR-alpha: Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl- 2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. Belongs to the nuclear hormone receptor family. NR1 subfamily.
Protein type: DNA-binding; Nuclear receptor
Chromosomal Location of Human Ortholog: 15 E2|15 40.42 cM
Cellular Component: nucleoplasm; nucleus
Molecular Function: DNA binding; DNA binding transcription factor activity; drug binding; ligand-dependent nuclear receptor activity; lipid binding; metal ion binding; NFAT protein binding; phosphatase binding; protein binding; protein complex binding; protein domain specific binding; protein heterodimerization activity; receptor activity; sequence-specific DNA binding; steroid hormone receptor activity; transcription factor binding; ubiquitin conjugating enzyme binding; zinc ion binding
Biological Process: behavioral response to nicotine; circadian regulation of gene expression; epidermis development; fatty acid metabolic process; glucose metabolic process; lipid metabolic process; lipoprotein metabolic process; negative regulation of appetite; negative regulation of blood pressure; negative regulation of cholesterol storage; negative regulation of inflammatory response; negative regulation of macrophage derived foam cell differentiation; negative regulation of neuron death; negative regulation of protein binding; negative regulation of receptor biosynthetic process; negative regulation of sequestering of triglyceride; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription regulatory region DNA binding; positive regulation of fatty acid oxidation; positive regulation of gluconeogenesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; regulation of cellular ketone metabolic process by positive regulation of transcription from RNA polymerase II promoter; regulation of circadian rhythm; regulation of fatty acid metabolic process; regulation of gene expression; regulation of glycolytic process by positive regulation of transcription from RNA polymerase II promoter; regulation of lipid transport by positive regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; response to hypoxia; response to insulin stimulus; rhythmic process; steroid hormone mediated signaling; transcription, DNA-dependent; wound healing
Research Articles on PPARa
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Product Notes
The PPARa ppara (Catalog #AAA2028796) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Peroxisome Proliferator Activated Receptor Alpha (PPARa) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Peroxisome Proliferator Activated Receptor Alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the PPARa ppara for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Peroxisome Proliferator Activated Receptor Alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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