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Western Blot (WB)
(Western Blot: Sample: Rat Spleen lysate; Primary Ab: 2ug/ml Rabbit Anti-Rat SIT1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Mouse DNA Fragmentation Factor Subunit Alpha Polyclonal Antibody | anti-DFFa antibody
Polyclonal Antibody to DNA Fragmentation Factor Subunit Alpha (DFFa)
Gene Names
Dffa; ICAD; DFF35; Dff45; ICAD-L; ICAD-S; A330085O09Rik
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
DNA Fragmentation Factor Subunit Alpha; Polyclonal Antibody; Polyclonal Antibody to DNA Fragmentation Factor Subunit Alpha (DFFa); anti-DFFa antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against DFFa. It has beenselected for its ability to recognize DFFa in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence Length
268
Applicable Applications for anti-DFFa antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
DFFa (Lys19~Ser262)
Organism Species
Mus musculus (Mouse)
Cross Reactivity
Human, Mouse
Conjugate
No Conjugate
Immunogen
Recombinant DFFa (Lys19~Ser262) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2082838
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Rat Spleen lysate; Primary Ab: 2ug/ml Rabbit Anti-Rat SIT1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Rat Spleen lysate; Primary Ab: 2ug/ml Rabbit Anti-Rat SIT1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Stomach Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Stomach Tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Lung Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Lung Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29,183 Da
NCBI Official Full Name
DNA fragmentation factor subunit alpha isoform a
NCBI Official Synonym Full Names
DNA fragmentation factor, alpha subunit
NCBI Official Symbol
Dffa
NCBI Official Synonym Symbols
ICAD; DFF35; Dff45; ICAD-L; ICAD-S; A330085O09Rik
NCBI Protein Information
DNA fragmentation factor subunit alpha
UniProt Protein Name
DNA fragmentation factor subunit alpha
Protein Family
UniProt Gene Name
Dffa
UniProt Synonym Gene Names
Icad; DFF-45; ICAD
Uniprot Description
DFFA: Inhibitor of the caspase-activated DNase (DFF40). Heterodimer of DFFA and DFFB. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Hydrolase
Chromosomal Location of Human Ortholog: 4|4 E2
Cellular Component: cytoplasm; cytosol; intracellular; nuclear chromatin; nucleus; plasma membrane
Molecular Function: protein binding; protein domain specific binding
Biological Process: apoptosis; negative regulation of deoxyribonuclease activity; negative regulation of execution phase of apoptosis; positive regulation of apoptosis
Research Articles on DFFa
Similar Products
Product Notes
The DFFa dffa (Catalog #AAA2028659) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to DNA Fragmentation Factor Subunit Alpha (DFFa) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DNA Fragmentation Factor Subunit Alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the DFFa dffa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNA Fragmentation Factor Subunit Alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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