Mouse Pyruvate Dehydrogenase Kinase Isozyme 4 (PDK4) ELISA Kit | PDK4 elisa kit

Mouse Pyruvate Dehydrogenase Kinase Isozyme 4 (PDK4) ELISA Kit

Cat. Num. AAA2024988

• Reactivity: Mouse
• Synonyms: Pyruvate Dehydrogenase Kinase Isozyme 4 (PDK4); Mouse Pyruvate Dehydrogenase Kinase Isozyme 4 (PDK4) ELISA Kit; [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4; mitochondrial; PDK4 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Pyruvate Dehydrogenase Kinase Isozyme 4 (PDK4).; No significant cross-reactivity or interference between Pyruvate Dehydrogenase Kinase Isozyme 4 (PDK4) and analogues was observed.
• Sequence Length: 411

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.127ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pyruvate Dehydrogenase Kinase Isozyme 4 (PDK4) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pyruvate Dehydrogenase Kinase Isozyme 4 (PDK4) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PDK4 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Dehydrogenase Kinase Isozyme 4 (PDK4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pyruvate Dehydrogenase Kinase Isozyme 4 (PDK4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Dehydrogenase Kinase Isozyme 4 (PDK4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pyruvate Dehydrogenase Kinase Isozyme 4 (PDK4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PDK4 elisa kit

Metabolic pathway; Cardiovascular biology

NCBI and Uniprot Product Information

• NCBI GI #: 4505693
• NCBI GeneID: 5166
• NCBI Accession #: NP_002603.1
• NCBI GenBank Nucleotide #: NM_002612.3
• Molecular Weight: 46,469 Da
• NCBI Official Full Name: pyruvate dehydrogenase kinase, isozyme 4
• NCBI Official Synonym Full Names: pyruvate dehydrogenase kinase, isozyme 4
• NCBI Official Symbol: PDK4
• NCBI Protein Information: pyruvate dehydrogenase kinase, isozyme 4
• UniProt Protein Name: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial
• UniProt Gene Name: PDK4
• UniProt Synonym Gene Names: PDHK4
• UniProt Entry Name: PDK4_HUMAN

NCBI Description

This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin. [provided by RefSeq, Jul 2008]

Uniprot Description

PDHK4: an ubiquitously expressed, atypical protein kinase associated with the mitochondrial matrix. The PDHKs play crucial roles in switching metabolic flux from oxidative phosphorylation towards glycolysis. PDHK4 is abundant in heart, skeletal muscle, kidney, and pancreatic islets. Contains a HATPase_c catalytic domain, found in several ATP-binding proteins including protein histidine kinases (PHKs), PHDKs, DNA gyrase B, topoisomerases, heat shock proteins, and DNA mismatch repair proteins. PDHK regulates glucose oxidation through inhibitory phosphorylation of the E1 alpha subunit of the mitochondrial pyruvate dehydrogenase complex (PDHC) at any one of 3 inhibitory serine residues. Inhibitory sites 1, 2, and 3 correspond to S293, S300, and S232 in human PDHA1, respectively. Four PDHK isoenzymes have been described, each with different site specificity: all four phosphorylate sites 1 and 2 but at different rates; for site 1 PDHK2 >PDHK4 >PDHK1 >PDHK3; for site 2, PDHK3> PDHK4 > PDHK2 > PDHK1. Only PDHK1 phosphorylates site 3. PDHKs are recruited to the PDHC by binding to a lipoyl group covalently attached to the inner lipoyl domain of the E2 component. PDHA1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. Suppression of PDH by PDHK inhibits the conversion of pyruvate to acetyl-CoA, attenuates mitochondrial respiration, and may contribute to the increased lactate production observed in many tumors. The PDH pathway is repressed in a majority of non-small cell lung carcinomas. Inhibited by AZD7545, dichloroacetate (DCA), and radicicol. Radicicol inhibits kinase activity by binding directly to the ATP-binding pocket of PDHK, similar to HSP90 from the same ATPase/kinase superfamily.

Protein type: Kinase, protein; Mitochondrial; EC 2.7.11.2; Protein kinase, atypical; ATYPICAL group; PDHK family

Chromosomal Location of Human Ortholog: 7q21.3

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane

Molecular Function: pyruvate dehydrogenase (acetyl-transferring) kinase activity; ATP binding; protein kinase activity

Biological Process: regulation of bone resorption; glucose metabolic process; regulation of acetyl-CoA biosynthetic process from pyruvate; cellular response to starvation; glucose homeostasis; protein amino acid phosphorylation; response to starvation; cellular metabolic process; regulation of fatty acid biosynthetic process; insulin receptor signaling pathway; regulation of fatty acid oxidation; regulation of pH; pyruvate metabolic process