Mouse PDK4 ELISA Kit | PDK4 elisa kit

Mouse PDK4 (Pyruvate Dehydrogenase Kinase Isozyme 4) ELISA Kit

Cat. Num. AAA2500633

• Reactivity: Mouse
• Synonyms: PDK4; Mouse PDK4 (Pyruvate Dehydrogenase Kinase Isozyme 4) ELISA Kit; PDK4 elisa kit

• Reactivity: Mouse
• Specificity: This kit recognizes natural and recombinant Mouse PDK4. No significant cross-reactivity or interference between Mouse PDK4 and analogues was observed.
• Sequence Length: 411

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.313-20ng/mL
• Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL
• Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse PDK4 concentrations in serum, plasma and other biological fluids.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PDK4 elisa kit

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PDK4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PDK4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PDK4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of PDK4. You can calculate the concentration of PDK4 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4505693
• NCBI GeneID: 5166
• NCBI Accession #: NP_002603.1
• NCBI GenBank Nucleotide #: NM_002612.3
• UniProt Accession #: Q16654
• Molecular Weight: 46,469 Da
• NCBI Official Full Name: pyruvate dehydrogenase kinase, isozyme 4
• NCBI Official Synonym Full Names: pyruvate dehydrogenase kinase, isozyme 4
• NCBI Official Symbol: PDK4
• NCBI Protein Information: pyruvate dehydrogenase kinase, isozyme 4; [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial; pyruvate dehydrogenase kinase, isoenzyme 4; pyruvate dehydrogenase, lipoamide, kinase isozyme 4, mitochondrial
• UniProt Protein Name: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial
• UniProt Gene Name: PDK4
• UniProt Synonym Gene Names: PDHK4
• UniProt Entry Name: PDK4_HUMAN

NCBI Description

This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin. [provided by RefSeq, Jul 2008]

Uniprot Description

PDHK4: an ubiquitously expressed, atypical protein kinase associated with the mitochondrial matrix. The PDHKs play crucial roles in switching metabolic flux from oxidative phosphorylation towards glycolysis. PDHK4 is abundant in heart, skeletal muscle, kidney, and pancreatic islets. Contains a HATPase_c catalytic domain, found in several ATP-binding proteins including protein histidine kinases (PHKs), PHDKs, DNA gyrase B, topoisomerases, heat shock proteins, and DNA mismatch repair proteins. PDHK regulates glucose oxidation through inhibitory phosphorylation of the E1 alpha subunit of the mitochondrial pyruvate dehydrogenase complex (PDHC) at any one of 3 inhibitory serine residues. Inhibitory sites 1, 2, and 3 correspond to S293, S300, and S232 in human PDHA1, respectively. Four PDHK isoenzymes have been described, each with different site specificity: all four phosphorylate sites 1 and 2 but at different rates; for site 1 PDHK2 >PDHK4 >PDHK1 >PDHK3; for site 2, PDHK3> PDHK4 > PDHK2 > PDHK1. Only PDHK1 phosphorylates site 3. PDHKs are recruited to the PDHC by binding to a lipoyl group covalently attached to the inner lipoyl domain of the E2 component. PDHA1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. Suppression of PDH by PDHK inhibits the conversion of pyruvate to acetyl-CoA, attenuates mitochondrial respiration, and may contribute to the increased lactate production observed in many tumors. The PDH pathway is repressed in a majority of non-small cell lung carcinomas. Inhibited by AZD7545, dichloroacetate (DCA), and radicicol. Radicicol inhibits kinase activity by binding directly to the ATP-binding pocket of PDHK, similar to HSP90 from the same ATPase/kinase superfamily.

Protein type: Mitochondrial; Kinase, protein; EC 2.7.11.2; Protein kinase, atypical; ATYPICAL group; PDHK family

Chromosomal Location of Human Ortholog: 7q21.3

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane

Molecular Function: pyruvate dehydrogenase (acetyl-transferring) kinase activity; ATP binding; protein kinase activity

Biological Process: regulation of bone resorption; glucose metabolic process; regulation of acetyl-CoA biosynthetic process from pyruvate; cellular response to starvation; glucose homeostasis; protein amino acid phosphorylation; response to starvation; cellular metabolic process; regulation of fatty acid biosynthetic process; regulation of fatty acid oxidation; insulin receptor signaling pathway; regulation of pH; pyruvate metabolic process