Human Response Gene To Complement 32 (RGC32) ELISA Kit | RGC32 elisa kit

Human Response Gene To Complement 32 (RGC32) ELISA Kit

Cat. Num. AAA2024143

• Gene Names: RGCC; RGC32; RGC-32; C13orf15; bA157L14.2
• Reactivity: Human
• Synonyms: Response Gene To Complement 32 (RGC32); Human Response Gene To Complement 32 (RGC32) ELISA Kit; RGCC; C13orf15; Regulator of cell cycle RGCC; RGC32 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Response Gene To Complement 32 (RGC32).; No significant cross-reactivity or interference between Response Gene To Complement 32 (RGC32) and analogues was observed.
• Sequence Length: 117

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.128ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Response Gene To Complement 32 (RGC32) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Response Gene To Complement 32 (RGC32) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RGC32 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Response Gene To Complement 32 (RGC32). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Response Gene To Complement 32 (RGC32). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Response Gene To Complement 32 (RGC32), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Response Gene To Complement 32 (RGC32) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for RGC32 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 119629069
• NCBI GeneID: 28984
• UniProt Accession #: Q9H4X1; Q6NZ48; Q9UL69
• Molecular Weight: 12,924 Da
• NCBI Official Full Name: response gene to complement 32, isoform CRA_b
• NCBI Official Synonym Full Names: regulator of cell cycle
• NCBI Official Symbol: RGCC
• NCBI Official Synonym Symbols: RGC32; RGC-32; C13orf15; bA157L14.2
• NCBI Protein Information: regulator of cell cycle RGCC
• UniProt Protein Name: Regulator of cell cycle RGCC
• UniProt Gene Name: RGCC
• UniProt Synonym Gene Names: C13orf15; RGC32; RGC-32
• UniProt Entry Name: RGCC_HUMAN

NCBI Description

This gene is thought to regulate cell cycle progression. It is induced by p53 in response to DNA damage, or by sublytic levels of complement system proteins that result in activation of the cell cycle. The encoded protein localizes to the cytoplasm during interphase and to centrosomes during mitosis. The protein forms a complex with polo-like kinase 1. The protein also translocates to the nucleus in response to treatment with complement system proteins, and can associate with and increase the kinase activity of cell division cycle 2 protein. In different assays and cell types, overexpression of this protein has been shown to activate or suppress cell cycle progression. [provided by RefSeq, Jul 2008]

Uniprot Description

RGC32: modulates the activity of cell cycle-specific kinases. Interacts with CDK1 and PLK1. Enhances CDK1 activity and contribute to the regulation of the cell cycle. May inhibit growth of glioma cells by promoting arrest of mitotic progression at the G2/M transition. Fibrogenic factor contributing to the pathogenesis of renal fibrosis through fibroblast activation. Interacts with SMAD3. Cytoplasmic in unstimulated cells; translocates to the nucleus following activation by complement. Associated with the centrosome during prometaphase and metaphase. Induced by Epstein-Barr virus (EBV). Up-regulated in aorta endothelial cells in response to complement activation. Important in vascular smooth muscle cell (VSMC) differentiation following transforming growth factor-beta (TGF-beta) induction. Its expression is greatly induced by TGF-beta in neural crest cells, leading to the induction of VSMC gene promoters. Smad and RhoA signaling are important for RGC-32 activation in neural crest cells. Forms a complex with hnRNP A/B that regulates the transcription of VSMC differentiation genes. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, regulatory subunit; Cell cycle regulation

Chromosomal Location of Human Ortholog: 13q14.11

Cellular Component: centrosome; cytoplasm; nucleus

Molecular Function: protein binding; protein kinase binding; protein kinase activator activity

Biological Process: positive regulation of mitosis; positive regulation of collagen biosynthetic process; negative regulation of cytokine secretion; negative regulation of blood vessel endothelial cell migration; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; complement activation; negative regulation of cell proliferation; negative regulation of angiogenesis; negative regulation of exit from mitosis; positive regulation of stress fiber formation; negative regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; positive regulation of cytokine secretion