Typical Testing Data/Standard Curve (for reference only)
Human Nucleophosmin (NPM) ELISA Kit | NPM elisa kit
Human Nucleophosmin (NPM) ELISA Kit
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to nucleophosmin. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to nucleophosmin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain nucleophosmin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of nucleophosmin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Nov 2009]
Uniprot Description
NPM1: a nucleolar protein associated with nucleolar ribonucleoprotein structures and that binds single-stranded nucleic acids. Is a major component of template activating factor (TAF)-III. It plays a role in controlling centrosome duplication, and may function in the assembly and/or transport of the ribosome. Two alternatively spliced isoforms have been described.
Protein type: Translation; Oncoprotein; Nucleolus; RNA-binding
Chromosomal Location of Human Ortholog: 5q35.1
Cellular Component: nucleoplasm; centrosome; focal adhesion; membrane; cytoplasm; nucleolus; spindle pole centrosome; cytosol; nucleus; ribonucleoprotein complex
Molecular Function: NF-kappaB binding; protein binding; protein homodimerization activity; protein kinase inhibitor activity; histone binding; Tat protein binding; RNA binding; protein heterodimerization activity; transcription coactivator activity; unfolded protein binding; protein kinase binding; ribosomal small subunit binding; ribosomal large subunit binding
Biological Process: regulation of centriole replication; viral reproduction; positive regulation of translation; centrosome cycle; cell aging; nucleocytoplasmic transport; signal transduction; DNA repair; protein oligomerization; activation of NF-kappaB transcription factor; intracellular protein transport; negative regulation of cell proliferation; nucleosome assembly; protein localization; regulation of endodeoxyribonuclease activity; DNA replication-independent nucleosome assembly at centromere; ribosome assembly; response to stress; negative regulation of apoptosis
Research Articles on NPM
Similar Products
Product Notes
The Human NPM npm1 (Catalog #AAA2024060) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2024060 ELISA Kit recognizes Human NPM. It is sometimes possible for the material contained within the vial of "Nucleophosmin (NPM), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
