Typical Testing Data/Standard Curve (for reference only)
Bovine Defensin Beta 103A (DEFb103A) ELISA Kit | DEFb103A elisa kit
Bovine Defensin Beta 103A (DEFb103A) ELISA Kit
Gene Names
DEFB3; BNDB3; DEFB2; DEFB6; bnbd3; BNBD-3; BNBD-6; BNDB-3; BNDB-6; defb405; DEFB103A
Reactivity
Bovine
Synonyms
Defensin Beta 103A (DEFb103A); Bovine Defensin Beta 103A (DEFb103A) ELISA Kit; DEFB103; DEFB3; HBD-3; HBD3; HBP-3; HBP3; Beta-defensin 3; Defensin-like protein; DEFb103A elisa kit
Reactivity
Bovine
Specificity
This assay has high sensitivity and excellent specificity for detection of Defb3. No significant cross-reactivity or interference between Defb3 and analogues was observed.
Sequence Length
57
Samples
Serum, plasma or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
31.25-2000pg/mL
Sensitivity
<12.5pg/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Defb3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Defb3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for DEFb103A elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of Defb3 in bovine serum, plasma or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defb3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to Defb3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defb3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Defb3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defb3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to Defb3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defb3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Defb3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
6,325 Da
NCBI Official Full Name
Beta-defensin 3
NCBI Official Symbol
DEFB3
NCBI Official Synonym Symbols
BNDB3; DEFB2; DEFB6; bnbd3; BNBD-3; BNBD-6; BNDB-3; BNDB-6; defb405; DEFB103A
NCBI Protein Information
beta-defensin 3
UniProt Protein Name
Beta-defensin 3
Protein Family
UniProt Gene Name
DEFB3
UniProt Synonym Gene Names
BNDB3
Uniprot Description
Has bactericidal activity. Active against E.coli ML35 and S.aureus 502A.
Research Articles on DEFb103A
Similar Products
Product Notes
The Bovine DEFb103A defb3 (Catalog #AAA454735) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA454735 ELISA Kit recognizes Bovine DEFb103A. It is sometimes possible for the material contained within the vial of "Defensin Beta 103A (DEFb103A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
