Peroxisome Proliferator Activated Receptor Gamma (PPARg) Recombinant Protein | PPARg recombinant protein

Recombinant Peroxisome Proliferator Activated Receptor Gamma (PPARg)

Cat. Num. AAA2009190

• Gene Names: Pparg; Nr1c3; PPARgamma; PPAR-gamma; PPARgamma2; PPAR-gamma2
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Peroxisome Proliferator Activated Receptor Gamma (PPARg); Recombinant Peroxisome Proliferator Activated Receptor Gamma (PPARg); PPARg recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- FH YGVHACEGCK GFFRRTIRLK LIYDRCDLNC RIHKKSRNKC QYCRFQKCLA VGMSHNAIRF GRMPQAEKEK LLAEISSDID QLNPESADLR ALAKHLYDSY IKSFPLTKAK ARAILTGKTT DKS
• Sequence Length: 475

• Applicable Applications for PPARg recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Phe149~Ser273 (Accession # P37238) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 187960105
• NCBI GeneID: 19016
• NCBI Accession #: NP_001120802.1
• NCBI GenBank Nucleotide #: NM_001127330.1
• UniProt Accession #: P37238
• Molecular Weight: 15.9kDa
• NCBI Official Full Name: peroxisome proliferator-activated receptor gamma isoform 1
• NCBI Official Synonym Full Names: peroxisome proliferator activated receptor gamma
• NCBI Official Symbol: Pparg
• NCBI Official Synonym Symbols: Nr1c3; PPARgamma; PPAR-gamma; PPARgamma2; PPAR-gamma2
• NCBI Protein Information: peroxisome proliferator-activated receptor gamma; nuclear receptor subfamily 1 group C member 3; peroxisome proliferator activated receptor gamma 2; peroxisome proliferator activated receptor gamma 4
• UniProt Protein Name: Peroxisome proliferator-activated receptor gamma
• Protein Family: Peroxisome proliferator-activated receptor
• UniProt Gene Name: Pparg
• UniProt Synonym Gene Names: Nr1c3; PPAR-gamma
• UniProt Entry Name: PPARG_MOUSE

NCBI Description

This gene encodes a nuclear receptor protein belonging to the peroxisome proliferator-activated receptor (Ppar) family. The encoded protein is a ligand-activated transcription factor that is involved in the regulation of adipocyte differentiation and glucose homeostasis. The encoded protein forms a heterodimer with retinoid X receptors and binds to DNA motifs termed "peroxisome proliferator response elements" to either activate or inhibit gene expression. Mice lacking the encoded protein die at an embryonic stage due to severe defects in placental vascularization. When the embryos lacking this gene are supplemented with healthy placentas, the mutants survive to term, but succumb to lipodystrophy and multiple hemorrhages. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]

Uniprot Description

PPAR-gamma: a transcription factor, member of the nuclear hormone receptor superfamily. Receptor for hypolipidemic drugs and fatty acids. Preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage. Regulator of adipogenesis and lipid metabolism, modulates insulin sensitivity, cell proliferation and inflammation. Phosphorylated and inhibited by MAP kinase. Heterodimerizes with the retinoid X receptor. Interacts with NCOA6 coactivator, leading to a strong increase in transcription of target genes. Two splice-variant isoforms have been described.

Protein type: Nuclear receptor; DNA-binding

Cellular Component: perinuclear region of cytoplasm; cytoplasm; nucleus; cytosol

Molecular Function: ligand-dependent nuclear receptor activity; transcription activator binding; zinc ion binding; metal ion binding; WW domain binding; drug binding; alpha-actinin binding; protein phosphatase binding; transcription factor binding; retinoid X receptor binding; arachidonic acid binding; protein binding; enzyme binding; DNA binding; ligand-dependent nuclear receptor transcription coactivator activity; sequence-specific DNA binding; steroid hormone receptor activity; estrogen receptor binding; chromatin binding; transcription factor activity

Biological Process: fat cell differentiation; diet induced thermogenesis; regulation of fat cell differentiation; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; negative regulation of collagen biosynthetic process; cell maturation; rhythmic process; glucose homeostasis; response to lipid; positive regulation of oligodendrocyte differentiation; placenta development; response to food; long-chain fatty acid transport; response to light stimulus; cell fate commitment; transcription, DNA-dependent; monocyte differentiation; negative regulation of acute inflammatory response; regulation of circadian rhythm; regulation of transcription from RNA polymerase II promoter; negative regulation of telomerase activity; cellular response to insulin stimulus; lipoprotein transport; regulation of gene expression; negative regulation of cytokine production; brown fat cell differentiation; positive regulation of fat cell differentiation; steroid hormone mediated signaling; positive regulation of transcription factor activity; fatty acid oxidation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of phagocytosis, engulfment; transcription from RNA polymerase II promoter; response to dietary excess; regulation of lipid metabolic process; negative regulation of smooth muscle cell proliferation; low-density lipoprotein receptor biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; signal transduction; negative regulation of cell proliferation; epithelial cell differentiation; regulation of transcription, DNA-dependent; regulation of blood pressure; inflammatory response; caspase activation; response to retinoic acid; intracellular receptor-mediated signaling pathway; response to low density lipoprotein stimulus; white fat cell differentiation; positive regulation of fatty acid oxidation; negative regulation of cell growth