Human Cathepsin E (CTSE) ELISA Kit | CTSE elisa kit

Human Cathepsin E (CTSE) ELISA Kit

Cat. Num. AAA2023096

• Gene Names: CTSE; CATE
• Reactivity: Human
• Synonyms: Cathepsin E (CTSE); Human Cathepsin E (CTSE) ELISA Kit; CTS-E; CATE; CTSE elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cathepsin E (CTSE).; No significant cross-reactivity or interference between Cathepsin E (CTSE) and analogues was observed.
• Sequence Length: 363

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.057ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cathepsin E (CTSE) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cathepsin E (CTSE) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CTSE elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cathepsin E (CTSE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cathepsin E (CTSE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cathepsin E (CTSE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cathepsin E (CTSE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CTSE elisa kit

Enzyme & Kinase; Tumor immunity; Gastroenterology

NCBI and Uniprot Product Information

• NCBI GI #: 181194
• NCBI GeneID: 1510
• UniProt Accession #: P14091; Q5TZ01; Q5TZ02; Q9NY58; Q9UCE3; Q9UCE4
• Molecular Weight: 39,553 Da
• NCBI Official Full Name: cathepsin E
• NCBI Official Synonym Full Names: cathepsin E
• NCBI Official Symbol: CTSE
• NCBI Official Synonym Symbols: CATE
• NCBI Protein Information: cathepsin E
• UniProt Protein Name: Cathepsin E
• Protein Family: Cathepsin
• UniProt Gene Name: CTSE
• UniProt Entry Name: CATE_HUMAN

NCBI Description

The protein encoded by this gene is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease, which is a member of the peptidase A1 family, has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that does not appear to be involved in the digestion of dietary protein and is found in highest concentration in the surface of epithelial mucus-producing cells of the stomach. It is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers. It appears, therefore, to be an oncofetal antigen. Transcript variants utilizing alternative polyadenylation signals and two transcript variants encoding different isoforms exist for this gene. [provided by RefSeq, Aug 2015]

Uniprot Description

CTSE: May have a role in immune function. Probably involved in the processing of antigenic peptides during MHC class II-mediated antigen presentation. May play a role in activation-induced lymphocyte depletion in the thymus, and in neuronal degeneration and glial cell activation in the brain. Belongs to the peptidase A1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.23.34; Protease

Chromosomal Location of Human Ortholog: 1q31

Cellular Component: endosome

Molecular Function: protein homodimerization activity; aspartic-type endopeptidase activity

Biological Process: protein autoprocessing; digestion; antigen processing and presentation of exogenous peptide antigen via MHC class II; proteolysis