Mouse Midkine (MK) ELISA Kit | MK elisa kit

Mouse Midkine (MK) ELISA Kit

Cat. Num. AAA2022312

• Gene Names: MDK; MK; ARAP; NEGF2
• Reactivity: Mouse
• Synonyms: Midkine (MK); Mouse Midkine (MK) ELISA Kit; MDK; ARAP; NEGF2; Neurite Growth-Promoting Factor 2; Amphiregulin-associated protein; Midgestation and kidney protein; Neurite outgrowth-promoting factor 2; MK elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Midkine (MK).; No significant cross-reactivity or interference between Midkine (MK) and analogues was observed.
• Sequence Length: 87

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Midkine (MK) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Midkine (MK) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MK elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Midkine (MK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Midkine (MK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Midkine (MK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Midkine (MK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for MK elisa kit

Signal transduction; Tumor immunity; Developmental science

NCBI and Uniprot Product Information

• NCBI GI #: 396080281
• NCBI GeneID: 4192
• NCBI Accession #: NP_001257480.1
• NCBI GenBank Nucleotide #: NM_001270551.1
• UniProt Accession #: P21741; Q2LEK4; Q9UCC7
• Molecular Weight: 9,512 Da
• NCBI Official Full Name: midkine isoform a
• NCBI Official Synonym Full Names: midkine (neurite growth-promoting factor 2)
• NCBI Official Symbol: MDK
• NCBI Official Synonym Symbols: MK; ARAP; NEGF2
• NCBI Protein Information: midkine
• UniProt Protein Name: Midkine
• Protein Family: Midkine
• UniProt Gene Name: MDK
• UniProt Synonym Gene Names: MK1; NEGF2; MK; ARAP
• UniProt Entry Name: MK_HUMAN

NCBI Description

This gene encodes a member of a small family of secreted growth factors that binds heparin and responds to retinoic acid. The encoded protein promotes cell growth, migration, and angiogenesis, in particular during tumorigenesis. This gene has been targeted as a therapeutic for a variety of different disorders. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2012]

Uniprot Description

MDK: Developmentally regulated, secreted growth factor homologous to pleiotrophin (PTN), which has heparin binding activity. Binds anaplastic lymphoma kinase (ALK) which induces ALK activation and subsequent phosphorylation of the insulin receptor substrate (IRS1), followed by the activation of mitogen-activated protein kinase (MAPK) and PI3-kinase, and the induction of cell proliferation. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development. Belongs to the pleiotrophin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: cell projection; cytoplasm; extracellular region

Molecular Function: heparin binding; growth factor activity

Biological Process: response to drug; nervous system development; Notch signaling pathway; cell migration; behavioral fear response; dentate gyrus development; adrenal gland development; cerebellar granular layer development; short-term memory; positive regulation of transcription, DNA-dependent; response to glucocorticoid stimulus; signal transduction; positive regulation of cell division; response to wounding; cerebral cortex development; negative regulation of neuron apoptosis; cell differentiation; defecation; regulation of behavior