Mouse Follistatin Like Protein 3 (FSTL3) ELISA Kit | FSTL3 elisa kit

Mouse Follistatin Like Protein 3 (FSTL3) ELISA Kit

Cat. Num. AAA2021344

• Gene Names: FSTL3; FLRG; FSRP
• Reactivity: Mouse
• Synonyms: Follistatin Like Protein 3 (FSTL3); Mouse Follistatin Like Protein 3 (FSTL3) ELISA Kit; FLRG; FSRP; Follistatin-Related Protein; Secreted Glycoprotein; Follistatin-related gene protein; FSTL3 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of FSTL3. No significant cross-reactivity or interference between FSTL3 and analogues was observed.
• Sequence Length: 237

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.6-1,000pg/mL
• Sensitivity: < 6.4pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FSTL3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FSTL3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FSTL3 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FSTL3 in mouse serum, plasma and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to FSTL3. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to FSTL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FSTL3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FSTL3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for FSTL3 elisa kit

Reproductive science

NCBI and Uniprot Product Information

• NCBI GI #: 5031701
• NCBI GeneID: 10272
• NCBI Accession #: NP_005851.1
• NCBI GenBank Nucleotide #: NM_005860.2
• UniProt Accession #: O95633; A8K7E3
• Molecular Weight: 24,960 Da
• NCBI Official Full Name: follistatin-related protein 3
• NCBI Official Synonym Full Names: follistatin-like 3 (secreted glycoprotein)
• NCBI Official Symbol: FSTL3
• NCBI Official Synonym Symbols: FLRG; FSRP
• NCBI Protein Information: follistatin-related protein 3
• UniProt Protein Name: Follistatin-related protein 3
• Protein Family: Follistatin-related protein
• UniProt Gene Name: FSTL3
• UniProt Synonym Gene Names: FLRG
• UniProt Entry Name: FSTL3_HUMAN

NCBI Description

Follistatin-like 3 is a secreted glycoprotein of the follistatin-module-protein family. It may have a role in leukemogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

FSTL3: Isoform 1 or the secreted form is a binding and antagonizing protein for members of the TGF-beta family, such us activin, BMP2 and MSTN. Inhibits activin A-, activin B-, BMP2- and MSDT-induced cellular signaling; more effective on activin A than on activin B. Involved in bone formation; inhibits osteoclast differentiationc. Involved in hematopoiesis; involved in differentiation of hemopoietic progenitor cells, increases hematopoietic cell adhesion to fibronectin and seems to contribute to the adhesion of hematopoietic precursor cells to the bone marrow stroma. Isoform 2 or the nuclear form is probably involved in transcriptional regulation via interaction with MLLT10. A chromosomal aberration involving FSTL3 is found in a case of B-cell chronic lymphocytic leukemia. Translocation t(11;19)(q13;p13) with CCDN1. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Secreted; Oncoprotein; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p13

Cellular Component: nucleoplasm; Golgi apparatus; extracellular space; extracellular region; nucleus; secretory granule

Molecular Function: protein binding; fibronectin binding; activin binding

Biological Process: negative regulation of osteoclast differentiation; ossification; transcription, DNA-dependent; adrenal gland development; male gonad development; negative regulation of BMP signaling pathway; regulation of transcription from RNA polymerase II promoter; negative regulation of activin receptor signaling pathway; positive regulation of cell-cell adhesion; positive regulation of transcription from RNA polymerase II promoter; hemopoietic progenitor cell differentiation; spermatogenesis; kidney development; lung development