Typical Testing Data/Standard Curve (for reference only)
Rat Mucin 5 Subtype AC (MUC5AC) ELISA Kit | MUC5AC elisa kit
Rat Mucin 5 Subtype AC (MUC5AC) ELISA Kit
Gene Names
MUC5AC; TBM; leB; MUC5; mucin
Reactivity
Rat
Synonyms
Mucin 5 Subtype AC (MUC5AC); Rat Mucin 5 Subtype AC (MUC5AC) ELISA Kit; TBM; MUC5; LeB; Oligomeric Mucus/Gel-Forming; Mucin 5; Subtypes A And C; Tracheobronchial/Gastric; Gastric mucin; Lewis B blood group antigen; Major airway glycoprotein; MUC5AC elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of MUC5AC. No significant cross-reactivity or interference between MUC5AC and analogues was observed.
Sequence Length
5654
Samples
Rat tissue homogenates, cell lysates and other biological fluids
Detection Range
0.312-20ng/mL
Sensitivity
< 0.113ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MUC5AC were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MUC5AC were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MUC5AC elisa kit
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to MUC5AC. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to MUC5AC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MUC5AC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MUC5AC in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for MUC5AC elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
585,570 Da
NCBI Official Full Name
mucin-5AC
NCBI Official Synonym Full Names
mucin 5AC, oligomeric mucus/gel-forming
NCBI Official Symbol
MUC5AC
NCBI Official Synonym Symbols
TBM; leB; MUC5; mucin
NCBI Protein Information
mucin-5AC
UniProt Protein Name
Mucin-5AC
Protein Family
UniProt Gene Name
MUC5AC
UniProt Synonym Gene Names
TBM
UniProt Entry Name
MUC5A_HUMAN
Uniprot Description
MUC5AC iso1:
Chromosomal Location of Human Ortholog: 11p15.5
Cellular Component: Golgi lumen; fibril
Molecular Function: extracellular matrix structural constituent
Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; fibril organization and biogenesis; post-translational protein modification
Research Articles on MUC5AC
Similar Products
Product Notes
The Rat MUC5AC muc5ac (Catalog #AAA2020727) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2020727 ELISA Kit recognizes Rat MUC5AC. It is sometimes possible for the material contained within the vial of "Mucin 5 Subtype AC (MUC5AC), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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