Rat Mucin 5 Subtype AC (MUC5AC) ELISA Kit | MUC5AC elisa kit

Rat Mucin 5 Subtype AC (MUC5AC) ELISA Kit

Cat. Num. AAA2702410

• Gene Names: MUC5AC; TBM; leB; MUC5; mucin
• Reactivity: Rat
• Synonyms: Mucin 5 Subtype AC (MUC5AC); Rat Mucin 5 Subtype AC (MUC5AC) ELISA Kit; TBM; MUC5; LeB; Oligomeric Mucus/Gel-Forming; Mucin 5; Subtypes A And C; Tracheobronchial/Gastric; Gastric mucin; Lewis B blood group antigen; Major airway glycoprotein; MUC5AC elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mucin 5 Subtype AC (MUC5AC).; No significant cross-reactivity or interference between Mucin 5 Subtype AC (MUC5AC) and analogues was observed.
• Sequence Length: 5654

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.116ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mucin 5 Subtype AC (MUC5AC) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mucin 5 Subtype AC (MUC5AC) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MUC5AC elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mucin 5 Subtype AC (MUC5AC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mucin 5 Subtype AC (MUC5AC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mucin 5 Subtype AC (MUC5AC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mucin 5 Subtype AC (MUC5AC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 748983076
• NCBI GeneID: 4586
• NCBI Accession #: NP_001291288.1
• NCBI GenBank Nucleotide #: NM_001304359.1
• UniProt Accession #: P98088; O60460; O76065; Q13792; Q14425; Q658Q1; Q7M4S5; Q8N4M9; Q8WWQ3; Q8WWQ4; Q8WWQ5; A0A096LPK4
• Molecular Weight: 585,570 Da
• NCBI Official Full Name: mucin-5AC
• NCBI Official Synonym Full Names: mucin 5AC, oligomeric mucus/gel-forming
• NCBI Official Symbol: MUC5AC
• NCBI Official Synonym Symbols: TBM; leB; MUC5; mucin
• NCBI Protein Information: mucin-5AC
• UniProt Protein Name: Mucin-5AC
• Protein Family: Mucin
• UniProt Gene Name: MUC5AC
• UniProt Synonym Gene Names: TBM

Uniprot Description

MUC5AC iso1:

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: cytoplasm; extracellular space; Golgi lumen; plasma membrane

Molecular Function: extracellular matrix structural constituent

Biological Process: O-glycan processing; phosphoinositide-mediated signaling; stimulatory C-type lectin receptor signaling pathway