Caspase 12 (CASP12) Recombinant Protein | CASP12 recombinant protein

Recombinant Caspase 12 (CASP12)

Cat. Num. AAA2018316

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Caspase 12 (CASP12); Recombinant Caspase 12 (CASP12); CASP12 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MAAKRTHERD PIYKIKGLAK DMLDGVFDDL MDKNVLNGDE LLKIGEGASL ILSKAENLVE SFFEKTEMAG KIFAGHIANS DKQLSLQFPS DDEEDELQKM FTPSSASESR GKVEDEEMEV NVGVAHASHL MLTVPQGIQS TEVQDSLKLC SRDWFCTMKT ERAEEIYPVM EKEGRTRLAL IICNKKFDYL FDRDDAETDILNMKELLQNL GYSVVIKENL TAQEMETELM KFAGRPEHQS SDST
• Sequence Length: 420

• Applicable Applications for CASP12 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Met1~Thr244 (Accession # Q920D5) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 5.1
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 18426852
• NCBI GeneID: 156117
• NCBI Accession #: NP_569106.1
• NCBI GenBank Nucleotide #: NM_130422.1
• UniProt Accession #: Q920D5
• Molecular Weight: 31.4kDa
• NCBI Official Full Name: caspase-12
• NCBI Official Synonym Full Names: caspase 12
• NCBI Official Symbol: Casp12
• NCBI Protein Information: caspase-12; CASP-12
• UniProt Protein Name: Caspase-12
• Protein Family: Inactive caspase
• UniProt Gene Name: Casp12
• UniProt Synonym Gene Names: CASP-12
• UniProt Entry Name: CASPC_RAT

NCBI Description

cysteine-aspartic acid protease (caspase); involved with the terminal stage of apoptosis [RGD, Feb 2006]

Uniprot Description

CASP12: Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF. Detected in heart, kidney, liver, lung, pancreas, small intestine, spleen, stomach, thymus and testis. Belongs to the peptidase C14A family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.22.-

Cellular Component: intracellular membrane-bound organelle; endoplasmic reticulum; intracellular; nucleus; cytosol

Molecular Function: protein binding; protease binding; cysteine-type endopeptidase activity; endopeptidase activity; cysteine-type peptidase activity

Biological Process: response to nicotine; response to drug; response to inorganic substance; response to anoxia; response to iron(III) ion; apoptosis; unfolded protein response; positive regulation of apoptosis; positive regulation of caspase activity; proteolysis; response to organic nitrogen; regulation of apoptosis; protein autoprocessing; response to ethanol; regulation of inflammatory response; response to cold; aging