Chromobox Homolog 3 (CBX3) Recombinant Protein | CBX3 recombinant protein

Recombinant Chromobox Homolog 3 (CBX3)

Cat. Num. AAA2012148

• Gene Names: Cbx3; M32; HP1g
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Chromobox Homolog 3 (CBX3); Recombinant Chromobox Homolog 3 (CBX3); CBX3 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- MASNKTTLQK MGKKQNGKSK KVEEAEPEEF VVEKVLDRRV VNGKVEYFLK WKGFTDADNT WEPEENLDCP ELIEDFLNSQ KAGKEKDGTK RKSLSDSESD DSKSKKKRDA ADKPRGFARG LDPERIIGAT DSSGELMFLM KWKDSDEADL VLAKEANMKC PQIVIAFYEE RLTWHSCPED EAQ
• Sequence Length: 183

• Applicable Applications for CBX3 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Met1~Gln183 (Accession # P23198) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 21903394
• NCBI GeneID: 12417
• UniProt Accession #: P23198; Q921C4
• Molecular Weight: 22.4kDa
• NCBI Official Full Name: Chromobox protein homolog 3
• NCBI Official Synonym Full Names: chromobox 3
• NCBI Official Symbol: Cbx3
• NCBI Official Synonym Symbols: M32; HP1g
• NCBI Protein Information: chromobox protein homolog 3; HP1 gamma; modifier 2 protein; chromobox homolog 3; heterochromatin protein 1 gamma; heterochromatin protein 1 homolog gamma
• UniProt Protein Name: Chromobox protein homolog 3
• Protein Family: Chromobox protein
• UniProt Gene Name: Cbx3
• UniProt Synonym Gene Names: HP1 gamma
• UniProt Entry Name: CBX3_MOUSE

Uniprot Description

CBX3: Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Binds directly to CHAF1A. Interacts with histone H3 methylated at 'Lys-9'. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Interacts with LBR, INCENP, TRIM28/TIF1B, SUV420H1, SUV420H2 and SP100. Interacts with TIF1A. Interacts with MIS12 and DSN1. Can interact directly with CBX5 via the chromoshadow domain. Interacts with POGZ. Interacts with CHAMP1. Interacts with ASXL1.

Protein type: DNA-binding

Cellular Component: nucleoplasm; nuclear envelope; spindle; nuclear heterochromatin; nucleus; chromatin; chromocenter

Molecular Function: protein domain specific binding; identical protein binding; protein binding; enzyme binding

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; rhythmic process; chromatin modification; negative regulation of transcription, DNA-dependent