Rabbit HP1 gamma Polyclonal Antibody | anti-CBX3 antibody

HP1 gamma Rabbit pAb

Cat. Num. AAA4752016

• Gene Names: Cbx3; M32; HP1g
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, Immunoprecipitation
• Purity: Affinity purified
• Synonyms: HP1 gamma; Polyclonal Antibody; HP1 gamma Rabbit pAb; Chromobox protein homolog 3; HECH; HP1-GAMMA; HP1Hs-gamma; anti-CBX3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Affinity purified
• Form/Format: Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

• Applicable Applications for anti-CBX3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
• Application Notes: WB: 1:1000; IHC: 1:20-1:500; ICC/IF: 1:50; FC/FACS: 1:50; IP: 1:20
• Immunogen: A synthetic peptide of mouse HP1 gamma
• Conjugation: Unconjugated
• Modification: Unmodification
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot detection of HP1 gamma in K562,C6,3T3 cell lysates using HP1 gamma Rabbit pAb(1:1000 diluted). Predicted band size:21kDa. Observed band size:21kDa.)

Related Product Information for anti-CBX3 antibody

At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.[provided by RefSeq, Mar 2011]
Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Probably involved in the repression of many genes located in euchromatin, such as E2F1, MYC and CDC25A. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the methyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1 (PubMed:24413057). Mediates the recruitement of NIPBL to sites of DNA damage at double-strand breaks (DSBs).

NCBI and Uniprot Product Information

• NCBI GI #: 21903394
• NCBI GeneID: 12417
• UniProt Accession #: P23198; Q921C4
• Molecular Weight: 20,855 Da
• NCBI Official Full Name: Chromobox protein homolog 3
• NCBI Official Synonym Full Names: chromobox 3
• NCBI Official Symbol: Cbx3
• NCBI Official Synonym Symbols: M32; HP1g
• NCBI Protein Information: chromobox protein homolog 3; HP1 gamma; chromobox homolog 3; heterochromatin protein 1 gamma; heterochromatin protein 1 homolog gamma; modifier 2 protein
• UniProt Protein Name: Chromobox protein homolog 3
• Protein Family: Chromobox protein
• UniProt Gene Name: Cbx3
• UniProt Synonym Gene Names: HP1 gamma
• UniProt Entry Name: CBX3_MOUSE

Uniprot Description

CBX3: Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Binds directly to CHAF1A. Interacts with histone H3 methylated at 'Lys-9'. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Interacts with LBR, INCENP, TRIM28/TIF1B, SUV420H1, SUV420H2 and SP100. Interacts with TIF1A. Interacts with MIS12 and DSN1. Can interact directly with CBX5 via the chromoshadow domain. Interacts with POGZ. Interacts with CHAMP1. Interacts with ASXL1.

Protein type: DNA-binding

Cellular Component: nucleoplasm; spindle; nuclear envelope; nuclear heterochromatin; nucleus; chromatin; chromocenter

Molecular Function: identical protein binding; protein domain specific binding; protein binding; enzyme binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; rhythmic process; chromatin modification; negative regulation of transcription, DNA-dependent