Mouse Chromobox Homolog 3 (CBX3) RTU ELISA Kit | CBX3 rtu elisa kit

Mouse Chromobox Homolog 3 (CBX3) ELISA Kit

Cat. Num. AAA458163

• Gene Names: Cbx3; M32; HP1g
• Reactivity: Mouse
• Synonyms: Chromobox Homolog 3 (CBX3); Mouse Chromobox Homolog 3 (CBX3) ELISA Kit; HECH; HP1-GAMMA; HP1Hs-Gamma; HP1 Gamma Homolog; Drosophila; Heterochromatin protein 1 homolog gamma; Modifier 2 protein; CBX3 rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 183

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.046ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CBX3 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CBX3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CBX3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CBX3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CBX3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 21903394
• NCBI GeneID: 12417
• UniProt Accession #: P23198; Q921C4
• Molecular Weight: 20,855 Da
• NCBI Official Full Name: Chromobox protein homolog 3
• NCBI Official Synonym Full Names: chromobox 3
• NCBI Official Symbol: Cbx3
• NCBI Official Synonym Symbols: M32; HP1g
• NCBI Protein Information: chromobox protein homolog 3
• UniProt Protein Name: Chromobox protein homolog 3
• Protein Family: Chromobox protein
• UniProt Gene Name: Cbx3
• UniProt Synonym Gene Names: HP1 gamma

Uniprot Description

Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Probably involved in the repression of many genes located in euchromatin, such as E2F1, MYC and CDC25A. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the methyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1 (PubMed:24413057). Mediates the recruitement of NIPBL to sites of DNA damage at double-strand breaks (DSBs) ().