Protein Tyrosine Phosphatase Receptor Type C (PTPRC) Recombinant Protein | PTPRC recombinant protein

Recombinant Protein Tyrosine Phosphatase Receptor Type C (PTPRC)

Cat. Num. AAA2011971

• Gene Names: Ptprc; loc; B220; Cd45; L-CA; Ly-5; T200; CD45R; Lyt-4
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Protein Tyrosine Phosphatase Receptor Type C (PTPRC); Recombinant Protein Tyrosine Phosphatase Receptor Type C (PTPRC); PTPRC recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.; MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGSEF-DLGIPETPKP SCGDPAARKT LVSWPEPVSK PESASKPHGY VLCYKNNSEK CKSLPNNVTS FEVESLKPYK YYEVSLLAYV NGKIQRNGTA EKCNFHTKAD RPDKVNGMKT SRPTDNSINV TCGPPYETNG PKTFYILVVR SGGSFVTKYN KTNCQFYVDN LYYSTDYEFL VSFHNGVYEG DSVIRN
• Sequence Length: 1291

• Applicable Applications for PTPRC recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Asp371~Asn556 (Accession # P06800) with two N-terminal Tags, His-tag and S-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

NCBI and Uniprot Product Information

• NCBI GI #: 341942176
• NCBI GeneID: 19264
• UniProt Accession #: P06800; Q61812; Q61813; Q61814; Q61815; Q78EF1; E9QLT5
• Molecular Weight: 26.6kDa
• NCBI Official Full Name: Receptor-type tyrosine-protein phosphatase C
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, receptor type, C
• NCBI Official Symbol: Ptprc
• NCBI Official Synonym Symbols: loc; B220; Cd45; L-CA; Ly-5; T200; CD45R; Lyt-4
• NCBI Protein Information: receptor-type tyrosine-protein phosphatase C; lymphocyte antigen 5; leukocyte common antigen; lymphocyte common antigen
• UniProt Protein Name: Receptor-type tyrosine-protein phosphatase C
• Protein Family: Receptor-type tyrosine-protein phosphatase
• UniProt Gene Name: Ptprc
• UniProt Synonym Gene Names: Ly-5; L-CA; Ly-5
• UniProt Entry Name: PTPRC_MOUSE

Uniprot Description

CD45: a receptor type protein tyrosine phosphatase protein. Contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. The first PTPAse domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Specifically expressed in hematopoietic cells. An essential regulator of T- and B-cell antigen receptor signaling. Functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. Suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced isoforms have been described.

Protein type: Membrane protein, integral; EC 3.1.3.48; Motility/polarity/chemotaxis; Receptor protein phosphatase, tyrosine

Cellular Component: focal adhesion; cell surface; membrane; integral to plasma membrane; plasma membrane; integral to membrane; intracellular; cytosol; external side of plasma membrane

Molecular Function: heparan sulfate proteoglycan binding; heparin binding; protein binding; hydrolase activity; phosphoric monoester hydrolase activity; protein tyrosine phosphatase activity; protein kinase binding; phosphoprotein phosphatase activity

Biological Process: B cell proliferation; positive regulation of isotype switching to IgG isotypes; activation of MAPK activity; regulation of humoral immune response mediated by circulating immunoglobulin; positive regulation of T cell mediated cytotoxicity; regulation of cell cycle; protein amino acid dephosphorylation; T cell receptor signaling pathway; T cell proliferation; B cell receptor signaling pathway; leukocyte adhesion; positive regulation of MAPKKK cascade; negative regulation of T cell mediated cytotoxicity; positive regulation of B cell proliferation; positive regulation of T cell mediated immunity; response to gamma radiation; positive regulation of T cell proliferation; negative regulation of protein amino acid autophosphorylation; negative regulation of cytokine and chemokine mediated signaling pathway; positive regulation of gamma-delta T cell differentiation; defense response to virus; T cell differentiation; positive thymic T cell selection; regulation of B cell receptor signaling pathway; positive regulation of humoral immune response mediated by circulating immunoglobulin; negative regulation of peptidyl-tyrosine phosphorylation; substrate-bound cell migration, cell release from substrate; positive regulation of antigen receptor-mediated signaling pathway; negative thymic T cell selection; bone marrow development; heterotypic cell-cell adhesion; stem cell development; dephosphorylation; positive regulation of T cell differentiation; B cell differentiation; release of sequestered calcium ion into cytosol; regulation of B cell differentiation; negative regulation of protein kinase activity; hemopoietic progenitor cell differentiation; positive regulation of alpha-beta T cell proliferation; immunoglobulin biosynthetic process