C Reactive Protein (CRP) Recombinant Protein | CRP recombinant protein

Recombinant C Reactive Protein (CRP)

Cat. Num. AAA2011681

• Gene Names: Crp; Aa1249; Ac1262; Ab1-341; Ab2-196; Ac1-114; Ac2-069; Ba2-693
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: C Reactive Protein (CRP); Recombinant C Reactive Protein (CRP); CRP recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal tags, his-tag and T7 tag, its sequence is listed below.; MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSEF-H EDMSKQAFVF PGVSATAYVS LEAESKKPLE AFTVCLYAHA DVSRSFSIFS YATKTSFNEI LLFWTRGQGF SIAVGGPEIL FSASEIPEVP THICATWESA TGIVELWLDG KPRVRKSLQK GYIVGTNASI ILGQEQDSYG GGFDANQSLV GDIGDVNMWD FVLSPEQINA VYVGRVFSPN VLNWRALKYE THGDVFIKPQ LWPLTDCCES
• Sequence Length: 230

• Applicable Applications for CRP recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: His20~Ser230 (Accession # P48199) with two N-terminal tags, his-tag and T7 tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 8393197
• NCBI GeneID: 25419
• NCBI Accession #: NP_058792.1
• NCBI GenBank Nucleotide #: NM_017096.3
• UniProt Accession #: P48199; Q5BK94
• Molecular Weight: 27.1kDa
• NCBI Official Full Name: C-reactive protein
• NCBI Official Synonym Full Names: C-reactive protein, pentraxin-related
• NCBI Official Symbol: Crp
• NCBI Official Synonym Symbols: Aa1249; Ac1262; Ab1-341; Ab2-196; Ac1-114; Ac2-069; Ba2-693
• NCBI Protein Information: C-reactive protein; C-reactive protein, petaxin related; C-reactive protein member of the pentraxin family; C-reactive protein, member of the pentraxin family
• UniProt Protein Name: C-reactive protein
• Protein Family: Cryparin
• UniProt Gene Name: Crp
• UniProt Synonym Gene Names: Ptx1
• UniProt Entry Name: CRP_RAT

NCBI Description

glycoprotein of the acute phase response [RGD, Feb 2006]

Uniprot Description

Function: Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells

By similarity.

Cofactor: Binds 2 calcium ions per subunit

By similarity.

Subunit structure: Homopentamer. Pentaxin (or pentraxin) have a discoid arrangement of 5 non-covalently bound subunits. Two of the five chains form a dimer linked by two interchain disulfide bonds located in the C-terminal heptapeptide and specific to rat CRP.

Subcellular location: Secreted.

Tissue specificity: Found in plasma.

Post-translational modification: The last two cysteines are involved either in interchain disulfide bonds or in an intrachain bond.

Sequence similarities: Belongs to the pentaxin family.Contains 1 pentaxin domain.