B7-2/CD86 recombinant protein

Recombinant B7-2/CD86

Cat. Num. AAA201160

• Gene Names: CD86; B70; B7-2; B7.2; LAB72; CD28LG2
• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: B7-2/CD86; Recombinant B7-2/CD86; CD86; B7-2; B7.2; B70; CD28LG2; LAB72; T-lymphocyte activation antigen CD86; Activation B7-2 antigen; BU63; CTLA-4 counter-receptor B7.2; FUN-1; B7-2/CD86 recombinant protein

• Host: Baculovirus
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid; In Phosphate Buffered Saline (pH 7.4) Containing 10% Glycerol.
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 31-247aa
• Sequence: YFNETADLPC QFANSQNQSL SELVVFWQDQ ENLVLNEVYL GKEKFDSVHS KYMGRTSFDS DSWTLRLHNL QIKDKGLYQC IIHHKKPTGM IRIHQMNSEL SVLANFSQPE IVPISNITEN VYINLTCSSI HGYPEPKKMS VLLRTKNSTI EYDGIMQKSQ DNVTELYDVS ISLSVSFPDV TSNMTIFCIL ETDKTRLLSS PFSIELEDPQ PPPDHIP HHH HHH
• Sequence Length: 329

• Applicable Applications for B7-2/CD86 recombinant protein: SDS-PAGE
• Species: Human
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Tag: His-Tag
• Predicted N-Terminal: Tyr 31
• Preparation and Storage: Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )

Related Product Information for B7-2/CD86 recombinant protein

B7-2/CD86, also known as T-lymphocyte activation antigen CD86 isoform 1, is a variably glycosylated protein in the B7 family. These family members play important roles in immune activation and the maintenance of immune tolerance. It is expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival and exists predominantly as a monomer on cell surfaces. Also, it interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells that it induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. Recombinant Human B7-2/CD86, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for B7-2/CD86 recombinant protein

Immunology

References

Grujic M., et al, (2010) Journal of Immunology. 185 (3): 1730-1743. Kuchroo VK., et al, (1995) Cell 80:707-718.

NCBI and Uniprot Product Information

• NCBI GI #: 317373339
• NCBI GeneID: 942
• UniProt Accession #: P42081
• Molecular Weight: 25.6kDa (223aa)
• NCBI Official Full Name: T-lymphocyte activation antigen CD86
• NCBI Official Synonym Full Names: CD86 molecule
• NCBI Official Symbol: CD86
• NCBI Official Synonym Symbols: B70; B7-2; B7.2; LAB72; CD28LG2
• NCBI Protein Information: T-lymphocyte activation antigen CD86
• UniProt Protein Name: T-lymphocyte activation antigen CD86
• UniProt Gene Name: CD86
• UniProt Synonym Gene Names: CD28LG2
• UniProt Entry Name: CD86_HUMAN

NCBI Description

This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]

Uniprot Description

CD86: Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T- cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Immunoglobulin superfamily; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q21

Cellular Component: cell surface; intracellular membrane-bound organelle; plasma membrane; integral to membrane; external side of plasma membrane

Molecular Function: protein binding; coreceptor activity; receptor activity; receptor binding

Biological Process: positive regulation of lymphotoxin A biosynthetic process; negative regulation of T cell anergy; T cell activation; viral reproduction; nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; positive regulation of interleukin-2 biosynthetic process; myeloid dendritic cell differentiation; positive regulation of activated T cell proliferation; positive regulation of interleukin-4 biosynthetic process; positive regulation of T-helper 2 cell differentiation; cell-cell signaling; T cell proliferation during immune response; positive regulation of cell proliferation; response to yeast; defense response to virus; aging; response to drug; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; B cell activation; T cell costimulation; toll-like receptor signaling pathway; innate immune response; immune response