Ferritin, Heavy Polypeptide (FTH) Recombinant Protein | FTH recombinant protein

Recombinant Ferritin, Heavy Polypeptide (FTH)

Cat. Num. AAA2011156

• Gene Names: Fth1; FHC; Fth; HFt; MFH
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Ferritin; Heavy Polypeptide (FTH); Recombinant Ferritin; FTH recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-MTTASPSQVR QNYHQDAEAA INRQINLELY ASYVYLSMSC YFDRDDVALK NFAKYFLHQS HEEREHAEKL MKLQNQRGGR IFLQDIKKPD RDDWESGLNA MECALHLEKS VNQSLLELHK LATDKNDPHL CDFIETYYLS EQVKSIKELG DHVTNLRKMG APEAGMAEYL FDKHTLGHGD ES
• Sequence Length: 182

• Applicable Applications for FTH recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Met1~Ser182 (Accession # P09528) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6753912
• NCBI GeneID: 14319
• NCBI Accession #: NP_034369.1
• NCBI GenBank Nucleotide #: NM_010239.2
• UniProt Accession #: P09528; Q3UI44
• Molecular Weight: 22.6kDa
• NCBI Official Full Name: ferritin heavy chain
• NCBI Official Synonym Full Names: ferritin heavy chain 1
• NCBI Official Symbol: Fth1
• NCBI Official Synonym Symbols: FHC; Fth; HFt; MFH
• NCBI Protein Information: ferritin heavy chain; H-ferritin; ferritin H subunit
• UniProt Protein Name: Ferritin heavy chain
• UniProt Gene Name: Fth1
• UniProt Synonym Gene Names: Fth; Ferritin H subunit
• UniProt Entry Name: FRIH_MOUSE

Uniprot Description

Ferritin-H: Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited. Belongs to the ferritin family.

Protein type: Oxidoreductase; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; EC 1.16.3.1

Cellular Component: mitochondrion; nucleus

Molecular Function: ferroxidase activity; ferric iron binding; metal ion binding; oxidoreductase activity

Biological Process: negative regulation of fibroblast proliferation; cellular iron ion homeostasis; iron ion transport; intracellular sequestering of iron ion