Mouse Ferritin heavy chain ELISA Kit | Fth1 elisa kit

Mouse Ferritin heavy chain ELISA Kit

Cat. Num. AAA288729

• Gene Names: Fth1; FHC; Fth; HFt; MFH
• Reactivity: Mouse
• Synonyms: Ferritin heavy chain; Mouse Ferritin heavy chain ELISA Kit; Ferritin H subunit; Fth1; Fth; 1.16.3.1; Fth1 elisa kit

• Reactivity: Mouse
• Specificity: Recombinant and natural Mouse Ferritin heavy chain
• Sequence Length: 182

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 3.12- 200 ng/mL
• Sensitivity: <1.6 ng/mL
• Intra-assay Precision: <=5.4%
• Inter-assay Precision: <=7.8%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Fth1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6753912
• NCBI GeneID: 14319
• NCBI Accession #: NP_034369.1
• NCBI GenBank Nucleotide #: NM_010239.2
• UniProt Accession #: P09528; Q3UI44
• Molecular Weight: 21,067 Da
• NCBI Official Full Name: ferritin heavy chain
• NCBI Official Synonym Full Names: ferritin heavy polypeptide 1
• NCBI Official Symbol: Fth1
• NCBI Official Synonym Symbols: FHC; Fth; HFt; MFH
• NCBI Protein Information: ferritin heavy chain
• UniProt Protein Name: Ferritin heavy chain
• Protein Family: Ferritin
• UniProt Gene Name: Fth1
• UniProt Synonym Gene Names: Fth; Ferritin H subunit
• UniProt Entry Name: FRIH_MOUSE

Uniprot Description

Ferritin-H: Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited. Belongs to the ferritin family.

Protein type: Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Oxidoreductase; EC 1.16.3.1

Cellular Component: mitochondrion; nucleus

Molecular Function: ferric iron binding; ferroxidase activity; metal ion binding; oxidoreductase activity

Biological Process: cellular iron ion homeostasis; intracellular sequestering of iron ion; iron ion transport; negative regulation of fibroblast proliferation