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Western Blot (WB) (Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 3ug/ml Mouse Anti-Mouse FTH Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )

Mouse anti-Mouse Ferritin, Heavy Polypeptide (FTH) Monoclonal Antibody | anti-FTH antibody

Monoclonal Antibody to Ferritin, Heavy Polypeptide (FTH)

Gene Names
Fth1; FHC; Fth; HFt; MFH
Reactivity
Mouse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Protein A + Protein G affinity chromatography
Synonyms
Ferritin; Heavy Polypeptide (FTH); Monoclonal Antibody; Monoclonal Antibody to Ferritin; FTH1; FTHL6; PIG15; PLIF; Ferritin Heavy Chain; Apoferritin; Placenta Immunoregulatory Factor; Proliferation-Inducing Protein 15; Cell proliferation-inducing gene 15 protein; anti-FTH antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2b Lambda
Clone Number
C1
Specificity
The antibody is a mouse monoclonal antibody raised against FTH. It has been selected for its ability to recognize FTH in immunohistochemical staining and western blotting.
Purity/Purification
Protein A + Protein G affinity chromatography
Form/Format
Liquid
Concentration
1mg/ml (varies by lot)
Sequence Length
182
Applicable Applications for anti-FTH antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
Western Blot: 0.5-5ug/mL
Immunocytochemistry in formalin fixed cells: 5-30ug/mL
Immunohistochemistry in formalin fixed frozen section: 5-30ug/mL
Immunohistochemistry in paraffin section: 5-30ug/mL
Immunogen
Recombinant Ferritin, Heavy Polypeptide (FTH)
Cross Reactivity
Mouse
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2069537
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 3ug/ml Mouse Anti-Mouse FTH Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )

Western Blot (WB) (Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 3ug/ml Mouse Anti-Mouse FTH Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant FTH, Mouse.)

Western Blot (WB) (Western Blot: Sample: Recombinant FTH, Mouse.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
21,067 Da
NCBI Official Full Name
ferritin heavy chain
NCBI Official Synonym Full Names
ferritin heavy polypeptide 1
NCBI Official Symbol
Fth1
NCBI Official Synonym Symbols
FHC; Fth; HFt; MFH
NCBI Protein Information
ferritin heavy chain
UniProt Protein Name
Ferritin heavy chain
UniProt Gene Name
Fth1
UniProt Synonym Gene Names
Fth; Ferritin H subunit

Uniprot Description

Ferritin-H: Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited. Belongs to the ferritin family.

Protein type: Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; EC 1.16.3.1; Oxidoreductase

Chromosomal Location of Human Ortholog: 19 A|19 6.23 cM

Cellular Component: autolysosome; extracellular exosome; mitochondrion; nucleus

Molecular Function: ferric iron binding; ferroxidase activity; iron ion binding; metal ion binding; oxidoreductase activity

Biological Process: cellular iron ion homeostasis; intracellular sequestering of iron ion; iron ion transport; negative regulation of fibroblast proliferation

Research Articles on FTH

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Product Notes

The FTH fth1 (Catalog #AAA2090561) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Ferritin, Heavy Polypeptide (FTH) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Ferritin, Heavy Polypeptide (FTH) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Western Blot: 0.5-5ug/mL Immunocytochemistry in formalin fixed cells: 5-30ug/mL Immunohistochemistry in formalin fixed frozen section: 5-30ug/mL Immunohistochemistry in paraffin section: 5-30ug/mL. Researchers should empirically determine the suitability of the FTH fth1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ferritin, Heavy Polypeptide (FTH), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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