Zeta Chain Associated Protein Kinase 70kDa (zAP70) Recombinant Protein | zAP70 recombinant protein

Recombinant Zeta Chain Associated Protein Kinase 70kDa (zAP70)

Cat. Num. AAA2010770

• Gene Names: Zap70; Srk; mur; mrtle; ZAP-70
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Zeta Chain Associated Protein Kinase 70kDa (zAP70); Recombinant Zeta Chain Associated Protein Kinase 70kDa (zAP70); zAP70 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-A HPSTFTQPQR RVDTLNSDGY TPEPARLASS TDKPRPMPMD TSVYESPYSD PEELKDKKLF LKRENLLVAD IELGCGNFGS VRQGVYRMRK KQIDVAIKVL KQGTEKADKD EMMREAQIMH QLDNPYIVRL IGVCQAEALM LVMEMAGGGP LHKFLLGKKE EIPVSNVAEL LHQVAMGMKY LEEKNFVHRD LAARNVLLVN RHYAKISDFG LSKALGADDS YYTARSAGKW PLKWYAPECI NFRKFSSRSD VWSYGVTMWE AFSYGQKPYK KMKGPEVLDF IKQGKRMECP
• Sequence Length: 618

• Applicable Applications for zAP70 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Ala270~Pro560 (Accession # P43404) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 31981916
• NCBI GeneID: 22637
• NCBI Accession #: NP_033565.2
• NCBI GenBank Nucleotide #: NM_009539.3
• UniProt Accession #: P43404; P97455; Q80VV2; Q8CHJ3
• Molecular Weight: 37.0kDa
• NCBI Official Full Name: tyrosine-protein kinase ZAP-70 isoform a
• NCBI Official Synonym Full Names: zeta-chain (TCR) associated protein kinase
• NCBI Official Symbol: Zap70
• NCBI Official Synonym Symbols: Srk; mur; mrtle; ZAP-70
• NCBI Protein Information: tyrosine-protein kinase ZAP-70; syk-related tyrosine kinase; 70 kDa zeta-chain associated protein
• UniProt Protein Name: Tyrosine-protein kinase ZAP-70
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: Zap70
• UniProt Synonym Gene Names: Srk; Zap-70
• UniProt Entry Name: ZAP70_MOUSE

NCBI Description

This gene encodes a member of the protein tyrosine kinase family. The encoded protein is essential for development of T lymphocytes and thymocytes, and functions in the initial step of T lymphocyte receptor-mediated signal transduction. A mutation in this gene causes chronic autoimmune arthritis, similar to rheumatoid arthritis in humans. Mice lacking this gene are deficient in alpha-beta T lymphocytes in the thymus. In humans, mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T lymphocytes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

ZAP70: a tyrosine kinase of the Syk family. Associates with the T-cell antigen receptor zeta-chain after TCR stimulation. Phosphorylated by Src-family kinases following antigen receptor activation. Plays a role in lymphocyte activation.

Protein type: Kinase, protein; Protein kinase, TK; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); TK group; Syk family

Cellular Component: T cell receptor complex; extrinsic to internal side of plasma membrane; membrane; cytoplasm; plasma membrane; immunological synapse; intercellular junction; intracellular; cytosol; lipid raft

Molecular Function: transferase activity; protein binding; phosphotyrosine binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; kinase activity; ATP binding; protein kinase activity; receptor binding

Biological Process: thymic T cell selection; cell migration; peptidyl-tyrosine phosphorylation; immune system process; protein amino acid autophosphorylation; positive regulation of calcium-mediated signaling; negative thymic T cell selection; T cell receptor signaling pathway; protein amino acid phosphorylation; positive regulation of T cell differentiation; innate immune response; positive regulation of alpha-beta T cell proliferation; immune response; beta selection; phosphorylation; positive regulation of alpha-beta T cell differentiation; transmembrane receptor protein tyrosine kinase signaling pathway; positive thymic T cell selection