M-CSF R/CD115 active protein

Recombinant M-CSF R/CD115

Cat. Num. AAA201060

• Gene Names: CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; BANDDOS; M-CSF-R
• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: M-CSF R/CD115; Recombinant M-CSF R/CD115; Macrophage colony-stimulating factor 1 receptor; CSF1R; C-FMS; CD115; CSF-1R; CSFR; FIM2; FMS; HDLS; M-CSF-R; M-CSF R/CD115 active protein

• Host: Baculovirus
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid; In Phosphate Buffered Saline (pH 7.4) Containing 10% Glycerol.
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 20-517aa
• Sequence: IPVIEPSVPE LVVKPGATVT LRCVGNGSVE WDGPPSPHWT LYSDGSSSIL STNNATFQNT GTYRCTEPGD PLGGSAAIHL YVKDPARPWN VLAQEVVVFE DQDALLPCLL TDPVLEAGVS LVRVRGRPLM RHTNYSFSPW HGFTIHRAKF IQSQDYQCSA LMGGRKVMSI SIRLKVQKVI PGPPALTLVP AELVRIRGEA AQIVCSASSV DVNFDVFLQH NNTKLAIPQQ SDFHNNRYQK VLTLNLDQVD FQHAGNYSCV ASNVQGKHST SMFFRVVESA YLNLSSEQNL IQEVTVGEGL NLKVMVEAYP GLQGFNWTYL GPFSDHQPEP KLANATTKDT YRHTFTLSLP RLKPSEAGRY SFLARNPGGW RALTFELTLR YPPEVSVIWT FINGSGTLLC AASGYPQPNV TWLQCSGHTD RCDEAQVLQV WDDPYPEVLS QEPFHKVTVQ SLLTVETLEH NQTYECRAHN SVGSGSWAFI PISAGAHTHP PDEFLFTPLE PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG KHHHHHH
• Sequence Length: 972

• Applicable Applications for M-CSF R/CD115 active protein: Bioactivity, SDS-PAGE
• Species: Human
• Endotoxin: <1.0 EU per 1ug of protein (determined by LAL method)
• Tag: hIgG-His-Tag
• Predicted N-Terminal: Ile20
• Biological Activity: Measured by its ability to inhibit M-CSF dependent proliferation of M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is less or equal to 100ng/ml in the presence of 10 ng/ml M-CSF.
• Preparation and Storage: Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )

Related Product Information for M-CSF R/CD115 active protein

CSF1R, also known as macrophage colony-stimulating factor 1 receptor, is a member of the type 3 subfamily of receptor tyrosine kinases. It is expressed primarily on cells of the monocyte and macrophage lineage, stem cells, and in the developing placenta and mediates most of the biological effects of this cytokine. This protein is consisted by an extracellular ligand-binding domain, a single membrane-spanning segment, and an intracellular tyrosine kinase domain. CSF1 and this receptor were initially implicated as essential for normal monocyte development as well as for trophoblastic implantation. This apparent role of CSF1/CSF1R in normal mammary gland development is very intriguing because this relationship has also been found in the biology of breast cancer in which abnormal expression of CSF1 and its receptor. Also, increased levels of CSF1R are found in microglia in Alzheimer's disease and after brain injuries. The increased receptor expression causes microglia to become more active. Recombinant human CSF1R, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for M-CSF R/CD115 active protein

Cardiovascular; Angiogenesis; Hormones & Cytokines

References

Yeung YG., et al, (1998) J Biol Chem. 273:17128-17137. Sapi E., et al, (1999) Proc Soc Exp Biol Med. 220:1-8.

NCBI and Uniprot Product Information

• NCBI GI #: 27262659
• NCBI GeneID: 1436
• NCBI Accession #: NP_005202.2
• NCBI GenBank Nucleotide #: NP_005202.2
• UniProt Accession #: P07333
• Molecular Weight: 82.1kDa (737aa); 70-100kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: macrophage colony-stimulating factor 1 receptor
• NCBI Official Synonym Full Names: colony stimulating factor 1 receptor
• NCBI Official Symbol: CSF1R
• NCBI Official Synonym Symbols: FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; BANDDOS; M-CSF-R
• NCBI Protein Information: macrophage colony-stimulating factor 1 receptor
• UniProt Protein Name: Macrophage colony-stimulating factor 1 receptor
• UniProt Gene Name: CSF1R
• UniProt Synonym Gene Names: FMS; CSF-1-R; CSF-1R; M-CSF-R
• UniProt Entry Name: CSF1R_HUMAN

NCBI Description

The protein encoded by this gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in this gene have been associated with a predisposition to myeloid malignancy. The first intron of this gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. Alternative splicing results in multiple transcript variants. Expression of a splice variant from an LTR promoter has been found in Hodgkin lymphoma (HL), HL cell lines and anaplastic large cell lymphoma. [provided by RefSeq, Mar 2017]

Uniprot Description

CSFR: an oncogenic tyrosine kinase receptor for CSF-1 (M-CSF). Drives growth and development of monocytes. Binding of CSF-1 induces receptor dimerization, activation and autophosphorylation of cytoplasmic tyrosine residues used as docking sites for SH2-containing signaling proteins. There are at least five major tyrosine autophosphorylation sites. Two point mutations seen in 10-20% of patients with acute myeloid leukemia, chronic myelomonocytic leukemia or myelodysplasia. One mutation appears to be both somatic and germline, and disrupts Cbl binding and receptor turnover. v-fms lacks the Cbl binding site and causes feline leukemia. Mutations may also develop after chemotherapy for lymphoma. A distinct point mutation was found in some cases of hepatocellular carcinoma and related to increased expression, and another mutation was found in 2 of 40 patients with idiopathic myelofibrosis. Expression is elevated in breast tumors and cell lines, and expression in xenografts and transgenic mice has been correlated with xenograft growth and breast cancer development. Inhibitors: Ki-20227 and other Kit/PDGFR inhibitors.

Protein type: Protein kinase, TK; Protein kinase, tyrosine (receptor); Oncoprotein; Kinase, protein; EC 2.7.10.1; Membrane protein, integral; TK group; PDGFR family

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: cell surface; integral to plasma membrane; plasma membrane; receptor complex

Molecular Function: macrophage colony stimulating factor receptor activity; protein homodimerization activity; cytokine binding; protein phosphatase binding; ATP binding

Biological Process: regulation of bone resorption; peptidyl-tyrosine phosphorylation; phosphoinositide-mediated signaling; macrophage differentiation; monocyte differentiation; multicellular organismal development; cytokine and chemokine mediated signaling pathway; protein amino acid autophosphorylation; osteoclast differentiation; signal transduction; positive regulation of tyrosine phosphorylation of Stat3 protein; cell proliferation; regulation of cell shape; positive regulation of cell proliferation; ruffle organization and biogenesis; innate immune response; hemopoiesis; phosphatidylinositol metabolic process; positive regulation of protein amino acid phosphorylation; inflammatory response; intercellular junction maintenance; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration

Disease: Leukoencephalopathy, Diffuse Hereditary, With Spheroids