V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (RELA) Recombinant Protein | RELA recombinant protein

Recombinant V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (RELA)

Cat. Num. AAA2009764

• Gene Names: Rela; p65
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (RELA); Recombinant V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (RELA); RELA recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSEF-GPY VEIIEQPKQR GMRFRYKCEG RSAGSIPGER STDTTKTHPT IKINGYTGPG TVRISLVTKD PPHRPHPHEL VGKDCRDGYY EADLCPDRSI HSFQNLGIQC VKKRDLEQAI SQRIQTNNNP FHVPIEEQRG DYDLNAVRLC FQVTVRDPAG RPLLLTPVLS HPIFDNRAPN TAELKICRVN RNSGSCLGGD EIFLLCDKVQ KEDIEVYFTG PGWEARGSFS QADVHRQVAI VFRTPPYADP SLQAPVRVSM QLRRPSDREL SEPMEFQYLP DTDDRHRIEE KRKRTY
• Sequence Length: 549

• Applicable Applications for RELA recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Gly18~Tyr306 (Accession # Q04207) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6677709
• NCBI GeneID: 19697
• NCBI Accession #: NP_033071.1
• NCBI GenBank Nucleotide #: NM_009045.4
• UniProt Accession #: Q04207; Q62025
• Molecular Weight: 36.8kDa
• NCBI Official Full Name: transcription factor p65
• NCBI Official Synonym Full Names: v-rel reticuloendotheliosis viral oncogene homolog A (avian)
• NCBI Official Symbol: Rela
• NCBI Official Synonym Symbols: p65
• NCBI Protein Information: transcription factor p65; p65 NFkB; p65 NF kappaB; p65 NF-kappa B; nuclear factor NF-kappa-B p65 subunit; avian reticuloendotheliosis viral (v-rel) oncogene homolog A; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
• UniProt Protein Name: Transcription factor p65
• Protein Family: Transcription factor
• UniProt Gene Name: Rela
• UniProt Synonym Gene Names: Nfkb3
• UniProt Entry Name: TF65_MOUSE

Uniprot Description

NFkB-p65: a subunit of NF-kappa-B transcription complex, which plays a crucial role in inflammatory and immune responses. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. P65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Three splice-variant isoforms have been identified.

Protein type: Nuclear receptor co-regulator; Transcription factor; DNA-binding

Cellular Component: nucleoplasm; transcription factor complex; protein complex; cytoplasm; nucleus; cytosol

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; identical protein binding; transcription activator binding; protein N-terminus binding; protein kinase binding; transcription factor binding; NF-kappaB binding; protein binding; enzyme binding; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; protein complex binding; chromatin binding; phosphate binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; cellular response to stress; positive regulation of transcription, DNA-dependent; negative regulation of insulin receptor signaling pathway; defense response; negative regulation of transcription from RNA polymerase II promoter; activation of NF-kappaB transcription factor; response to organic substance; hair follicle development; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; inflammatory response; positive regulation of Schwann cell differentiation; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; liver development; response to UV-B; regulation of transcription from RNA polymerase II promoter; organ morphogenesis; positive regulation of interleukin-12 biosynthetic process; positive regulation of chondrocyte differentiation; response to bacterium; response to muramyl dipeptide; response to cytokine stimulus; regulation of inflammatory response; innate immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of protein catabolic process; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis