Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (RELA) Polyclonal Antibody | anti-RELA antibody
Polyclonal Antibody to V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (RELA)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- PY VEIIEQPKQR GMRFRYKCEG RSAGSIPGER STDTTKTHPT IKINGYTGPG TVRISLVTKD PPHRPHPHEL VGKDCRDGYY EADLCPDRSI HSFQNLGIQC VKKRDLEQAI SQRIQTNNNP FHVPIEEQRG DYDLNAVRLC FQVTVRDPAG RPLLLTPVLS HPIFDNRAPN TAELKICRVN RNSGSCLGGD EIFLLCDKVQ KEDIEVYFTG PGWEARGSFS QADVHRQVAI VFRTPPYADP SLQAPVRVSM QLRRPSDREL SEPMEFQYLP DTDDRHRIEE KRKRTY
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Liver tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Liver tissue))
NCBI and Uniprot Product Information
Uniprot Description
NFkB-p65: a subunit of NF-kappa-B transcription complex, which plays a crucial role in inflammatory and immune responses. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. P65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Three splice-variant isoforms have been identified.
Protein type: DNA-binding; Nuclear receptor co-regulator; Transcription factor
Chromosomal Location of Human Ortholog: 19 A|19 4.34 cM
Cellular Component: cytoplasm; cytosol; NF-kappaB complex; nuclear chromatin; nucleus; protein complex; transcription factor complex
Molecular Function: actinin binding; activating transcription factor binding; ankyrin repeat binding; chromatin binding; chromatin DNA binding; DNA binding; DNA binding transcription factor activity; enzyme binding; histone deacetylase binding; identical protein binding; NF-kappaB binding; phosphate binding; protein binding; protein complex binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding; protein N-terminus binding; sequence-specific DNA binding; transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding; transcription factor binding; transcription regulatory region DNA binding; ubiquitin protein ligase binding
Biological Process: cellular response to hydrogen peroxide; cellular response to interleukin-1; cellular response to interleukin-6; cellular response to nicotine; cellular response to peptide hormone stimulus; cellular response to tumor necrosis factor; cytokine-mediated signaling pathway; defense response; hair follicle development; inflammatory response; innate immune response; liver development; negative regulation of apoptosis; negative regulation of extrinsic apoptotic signaling pathway; negative regulation of insulin receptor signaling pathway; negative regulation of NIK/NF-kappaB signaling; negative regulation of protein catabolic process; negative regulation of protein sumoylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; NIK/NF-kappaB signaling; nucleotide-binding oligomerization domain containing 2 signaling pathway; organ morphogenesis; positive regulation of cell proliferation; positive regulation of chondrocyte differentiation; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of interleukin-12 biosynthetic process; positive regulation of miRNA metabolic process; positive regulation of NF-kappaB transcription factor activity; positive regulation of NIK/NF-kappaB signaling; positive regulation of Schwann cell differentiation; positive regulation of T cell receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus; positive regulation of transcription, DNA-templated; regulation of DNA-templated transcription in response to stress; regulation of inflammatory response; regulation of NIK/NF-kappaB signaling; regulation of transcription, DNA-templated; response to bacterium; response to cytokine; response to muramyl dipeptide; response to muscle stretch; response to organic substance; response to UV-B; transcription, DNA-dependent; tumor necrosis factor-mediated signaling pathway
Research Articles on RELA
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Product Notes
The RELA rela (Catalog #AAA2001681) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (RELA) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (RELA) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the RELA rela for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- PY VEIIEQPKQR GMRFRYKCEG RSAGSIPGER STDTTKTHPT IKINGYTGPG TVRISLVTKD PPHRPHPHEL VGKDCRDGYY EADLCPDRSI HSFQNLGIQC VKKRDLEQAI SQRIQTNNNP FHVPIEEQRG DYDLNAVRLC FQVTVRDPAG RPLLLTPVLS HPIFDNRAPN TAELKICRVN RNSGSCLGGD EIFLLCDKVQ KEDIEVYFTG PGWEARGSFS QADVHRQVAI VFRTPPYADP SLQAPVRVSM QLRRPSDREL SEPMEFQYLP DTDDRHRIEE KRKRTY. It is sometimes possible for the material contained within the vial of "V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (RELA), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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