Regenerating Islet Derived Protein 3 Gamma (REG3g) Recombinant Protein | REG3g recombinant protein

Recombinant Regenerating Islet Derived Protein 3 Gamma (REG3g)

Cat. Num. AAA2009283

• Gene Names: Reg3g; AI449515
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Regenerating Islet Derived Protein 3 Gamma (REG3g); Recombinant Regenerating Islet Derived Protein 3 Gamma (REG3g); REG3g recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal tags, His-tag and S-tag, its sequence is listed below.; MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS - RAYGSY CYALFSVSKN WYDADMACQK RPSGHLVSVL SGAEASFLSS MIKSSGNSGQ YVWIGLHDPT LGYEPNRGGW EWSNADVMNY INWETNPSSS SGNHCGTLSR AS
• Sequence Length: 174

• Applicable Applications for REG3g recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Arg45~Ser152 (Accession # O09049) with two N-terminal tags, His-tag and S-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6755310
• NCBI GeneID: 19695
• NCBI Accession #: NP_035390.1
• NCBI GenBank Nucleotide #: NM_011260.1
• UniProt Accession #: O09049
• Molecular Weight: 17.3kDa
• NCBI Official Full Name: regenerating islet-derived protein 3-gamma
• NCBI Official Synonym Full Names: regenerating islet-derived 3 gamma
• NCBI Official Symbol: Reg3g
• NCBI Official Synonym Symbols: AI449515
• NCBI Protein Information: regenerating islet-derived protein 3-gamma; REG-3-gamma; reg III-gamma; RegIII (gamma); pancreatitis-associated protein 3; regenerating islet-derived protein III-gamma; generating islet-derived, mouse homolog 3 gamma
• UniProt Protein Name: Regenerating islet-derived protein 3-gamma
• Protein Family: Regenerating islet-derived protein
• UniProt Gene Name: Reg3g
• UniProt Synonym Gene Names: Pap3; REG-3-gamma
• UniProt Entry Name: REG3G_MOUSE

Uniprot Description

Function: Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Restricts bacterial colonization of the intestinal epithelial surface and consequently limits activation of adaptive immune responses by the microbiota. The uncleaved form has bacteriostatic activity, whereas the cleaved form has bactericidal activity against L.monocytogenes and methicillin-resistant S.aureus. Regulates keratinocyte proliferation and differentiation after skin injury. Ref.3 Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9

Subcellular location: Secreted. Cytoplasm

By similarity.

Tissue specificity: Predominantly expressed in the small intestine, including Paneth cells (at protein level). Hardly detectable in the colon (at protein level). Highly expressed in the lung epithelium during methicillin-resistant S.aureus infection (at protein level). Skin injury increases its epidermal expression. Also expressed in the pancreas. Ref.1 Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9

Developmental stage: In mid-small intestine, very low levels at birth. Expression levels rise dramatically during the weaning period (P17-P22) and remain high into adulthood in conventionally raised but not germfree animals. Ref.5

Induction: Up-regulated in Paneth cells by intestinal microbiota (at protein level). MyD88-mediated signals are essential for its induction in intestinal epithelial cells. Induction in the lung is dependent on IL6ST-induced STAT3 signaling. IL17A induces its expression in primary keratinocytes and skin wounds. Ref.5 Ref.6 Ref.8 Ref.9

Domain: The EPN motif is essential for recognition of the peptidoglycan carbohydrate backbone and for efficient bacterial killing with Glu-114 playing a key role in peptidoglycan binding and bactericidal activity

By similarity.

Post-translational modification: Proteolytic processing by trypsin removes an inhibitory N-terminal propeptide and is essential for peptidoglycan binding and antibacterial activity.

Disruption phenotype: Mutant mice are born at normal Mendelian ratios. They appear healthy and show no signs of enteropathy, but exhibit a marked increase in the number of mucosa-associated bacteria, predominantly Gram-positive, relative to cohoused wild-type littermates in distal small intestine. Ref.7

Sequence similarities: Contains 1 C-type lectin domain.