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Western Blot (WB) (Western Blot:Sample: Mouse Testis Tissue.)

Rabbit Regenerating Islet Derived Protein 3 Gamma (REG3g) Polyclonal Antibody | anti-REG3g antibody

Polyclonal Antibody to Regenerating Islet Derived Protein 3 Gamma (REG3g)

Gene Names
Reg3g; AI449515; REG-3-gamma; reg III-gamma
Reactivity
Mus musculus (Mouse)
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Regenerating Islet Derived Protein 3 Gamma (REG3g); Polyclonal Antibody; Polyclonal Antibody to Regenerating Islet Derived Protein 3 Gamma (REG3g); anti-REG3g antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mus musculus (Mouse)
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-REG3g antibody
Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP)
Application Notes
Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Source
Polyclonal antibody preparation
Traits
Liquid
Cross Reactivity
Rat
Immunogen
Recombinant REG3g (Arg45~Ser152) expressed in E.coli ( MBS2009283 ).
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.

Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot:Sample: Mouse Testis Tissue.)

Western Blot (WB) (Western Blot:Sample: Mouse Testis Tissue.)

Western Blot (WB)

(Western Blot:Sample: Recombinant REG3g, Mouse.)

Western Blot (WB) (Western Blot:Sample: Recombinant REG3g, Mouse.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P.Samples: Mouse Tissue)

Immunohistochemistry (IHC) (DAB staining on IHC-P.Samples: Mouse Tissue)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
regenerating islet-derived protein 3-gamma preproprotein
NCBI Official Synonym Full Names
regenerating islet-derived 3 gamma
NCBI Official Symbol
Reg3g
NCBI Official Synonym Symbols
AI449515; REG-3-gamma; reg III-gamma
NCBI Protein Information
regenerating islet-derived protein 3-gamma
UniProt Protein Name
Regenerating islet-derived protein 3-gamma
UniProt Gene Name
Reg3g
UniProt Synonym Gene Names
Pap3; REG-3-gamma; Reg III-gamma

NCBI Description

This gene encodes a C-type lectin that demonstrates bactericidal activity. This gene is predominantly expressed in the distal small intestine where the encoded protein undergoes proteolytic processing by trypsin. Mice lacking the encoded protein exhibit altered mucus distribution, increased bacterial contact with the epithelium, and elevated inflammatory markers in the ileum, and low-grade inflammation. [provided by RefSeq, Jun 2016]

Uniprot Description

Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Restricts bacterial colonization of the intestinal epithelial surface and consequently limits activation of adaptive immune responses by the microbiota. The uncleaved form has bacteriostatic activity, whereas the cleaved form has bactericidal activity against L.monocytogenes and methicillin-resistant S.aureus. Regulates keratinocyte proliferation and differentiation after skin injury.

Research Articles on REG3g

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Product Notes

The REG3g reg3g (Catalog #AAA2001929) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Regenerating Islet Derived Protein 3 Gamma (REG3g) reacts with Mus musculus (Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's Regenerating Islet Derived Protein 3 Gamma (REG3g) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the REG3g reg3g for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Regenerating Islet Derived Protein 3 Gamma (REG3g), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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